Zinc surplus in yeast cells has been previously investigated thanks to transcriptomic studies by using traditionally Saccharomyces cerevisiae as a model. However, proteome response under zinc‐replete conditions needs to be further studied in yeast. For that reason, eight yeast strains from seven different species were inoculated in zinc‐depleted and zinc‐replete media. The quantitative and qualitative comparative label‐free proteomic analysis enabled the identification of between 2000 and 3000 proteins from each strain, and changes to the proteome ranged from 2.5% to 43.7% of identified proteins. Functional analysis (Blast2Go) has allowed the characterization of differentially abundant proteins. Common zinc‐responsive proteins have been detected for the eight strains such as oxidoreductases and transferases (increased in abundance) although more of the changes detected were not shared by all the strains tested. Zinc distribution under replete conditions has been analysed in cell wall fractions, and cytoplasm plus organelles (intracellular fraction), with the latter identified to be the main zinc reservoir. Additionally, the energy dispersive spectroscopy coupled to the scanning electron microscopy technique has permitted the visualization of zinc in the whole cell. Proteomic analysis revealed that while there were some shared responses, the non‐model yeast species also showed distinct proteomic profiles in zinc‐replete conditions, compared to S. cerevisiae, revealing new zinc‐responsive proteins in yeast.

中文翻译：

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酿酒酵母和非模式酵母菌对锌的差异分布和蛋白质组学响应。

通过转录组学研究，以前使用传统酿酒酵母对酵母细胞中的锌过剩进行了研究。作为一个模型。但是，需要在酵母中进一步研究锌充足条件下的蛋白质组反应。因此，在贫锌和富锌培养基中接种了来自七个不同物种的八种酵母菌株。定量和定性的无标记比较蛋白质组学分析能够鉴定出每个菌株2000至3000种蛋白质，蛋白质组的变化范围为所鉴定蛋白质的2.5％至43.7％。功能分析（Blast2Go）允许表征差异丰富的蛋白质。尽管检测到的更多变化并未被所有测试菌株共有，但已检测到八种菌株的常见锌响应蛋白，例如氧化还原酶和转移酶（含量增加）。锌在充足条件下的分布已在细胞壁部分，细胞质和细胞器（细胞内部分）中进行了分析，后者被确定为主要的锌储库。另外，与扫描电子显微镜技术耦合的能量色散光谱法已经使整个细胞中的锌可视化。蛋白质组学分析显示，尽管存在一些共同的反应，但与锌相比，非模型酵母菌种在充锌条件下也显示出不同的蛋白质组学特征小号。酿酒酵母，揭示酵母中新的锌反应蛋白。