The global demand for jute fibres in industrial and commercial applications makes jute (Corchorus sp.) a popular cash crop. Jute is an ideal eco friendly material for packaging and product manufacturing owing to its long, shiny, non-toxic, biodegradable bast fibres and high yield of cellulosic biomass produced per acre in cultivation. In order to match the increasing global demand for jute fibres, sustainable protection of jute cultivation from insect pests is essential. Lepidopteran pest resistant Bt (Bacillus thuringiensis) jute lines were developed by expressing the fused Bt-cry1Ab/Ac gene, driven by the CaMV35S constitutive promoter in the white jute (C. capsularis) cultiver- JRC321 and named as CBT (CaMV35S Bt). Molecular analyses confirmed the single copy integration of the Bt-cry1Ab/Ac gene in the transgenic CBT lines and 0.25 ng/mg of Bt-Cry1Ab/Ac δ-endotoxin production in the leaf tissue. The study involved evaluation of the insect resistance and the level of Bt-cry1Ab/Ac gene expression in different parts (leaf, stem, seed coat, and root) of the CBT lines in comparison with the previously developed, actin1 promoter driven Bt-cry1Ab/Ac gene containing, ABT lines (actin1 Bt). The CBT and ABT (Bt jute) lines were tested against two major jute pests- hairy caterpillars (Spilarctia obliqua) and indigo caterpillars (Spodoptera exigua). The Abbott’s insect mortality was observed for hairy caterpillars and indigo caterpillars on CBT lines as 90.74 % and 76.37 % respectively and on ABT lines as 97.11 % and 80.74 % respectively. Progeny plants of both the CBT and ABT lines showed similar and significant effects of insect resistance on the test insects under bioassay. Quantification of the expression of Bt-cry1Ab/Ac gene transcripts was similar in the leaves of both CBT and ABT lines but for stems, seed coats, and roots, the expression was found to be higher in ABT lines. This study confirmed that ABT lines were superior and more promising than CBT lines for jute pest management. The progeny plants from both CBT and ABT lines were similar to WT plants in terms of agronomic parameters.

全球在工业和商业应用中对黄麻纤维的需求使黄麻（Corchorus sp。）成为流行的经济作物。由于黄麻长，发亮，无毒，可生物降解的韧皮纤维以及每英亩耕地产生的纤维素生物量高产，黄麻是用于包装和产品制造的理想环保材料。为了满足全球对黄麻纤维不断增长的需求，对黄麻种植进行防虫害的可持续保护至关重要。通过表达融合的Bt - cry1Ab / Ac基因（由CaMV35S组成启动子驱动）在白麻（C.荚膜囊虫）中产生了鳞翅目害虫抗性Bt（苏云金芽孢杆菌）黄麻品系。）品种-JRC321，并命名为CBT（C aMV35S Bt）。分子分析证实了的单拷贝整合的Bt-cry1Ab基因/ AC基因在转基因CBT线和0.25纳克/毫克BT-的Cry1Ab / ACδ内毒素生产在叶组织中。与先前开发的actin1启动子驱动的Bt-cry1Ab相比，该研究涉及评估CBT系不同部位（叶，茎，种皮和根）的昆虫抗性和Bt-cry1Ab / Ac基因表达水平/ AC基因含有，ABT线（一ctin1 Bt基因）。CBT和ABT（Bt测试了黄麻品系对两种主要的黄麻害虫-毛毛虫（Splarctia obliqua）和靛蓝毛虫（Spodoptera exigua）。在CBT品系上观察到的毛毛虫和靛蓝毛虫的雅培昆虫死亡率分别为90.74％和76.37％，在ABT品系上分别为97.11％和80.74％。CBT和ABT品系的后代植物在生物测定法下均显示出抗虫性对受试昆虫具有相似而显着的影响。Bt-cry1Ab / Ac表达的定量CBT和ABT品系的叶子中的基因转录物相似，但是对于茎，种皮和根，发现在ABT品系中表达更高。这项研究证实，对于黄麻害虫管理，ABT品系优于CBT品系。就农艺学参数而言，来自CBT和ABT品系的后代植物与WT植物相似。