In this study, we performed a transcriptomic analysis to investigate genome-wide gene profiles of a rapeseed (Brassica napus) cultivar Zhongshuang 11 (ZS11) in response to waterlogging stress at germination stage. The root and shoot development of ZS11 was dramatically inhibited by waterlogging stress. As a result, 7472 downregulated DEGs (differentially expressed genes) and 3869 upregulated DEGs were identified by the RNA-seq experiments. KEGG analyses show that enriched pathways of these DEGs mainly include “plant hormone signal transduction,” “phenylpropanoid biosynthesis,” and “starch and sucrose metabolism.” Among them, there are 41, 19, and 26 DEGs involved in the regulatory pathways of root, shoot, and hypocotyl growth, respectively. In detail, the downregulated IAA17, IAA28, YUCCA9, UPBEAT1, and ARR1 and the upregulated ACR4 probably affect the balance between cell differentiation and division in the root apical meristem (RAM), while the downregulated CLV1, CRN, AHK4, and FAS2 might disturb stem cell fate in the shoot apical meristem (SAM). Taken together, the current results provide valuable information for understanding molecular mechanisms of seedling development in response to waterlogging stress at germination stage in rapeseed.

在这项研究中，我们进行了转录组学分析，以研究油菜（中小油菜）品种中双11（ZS11）在萌发阶段对涝渍胁迫的响应的全基因组基因谱。淹水胁迫显着抑制了ZS11的根和芽发育。结果，通过RNA-seq实验鉴定了7472个DEGs（差异表达基因）和3869个DEGs。KEGG分析表明，这些DEG的丰富途径主要包括“植物激素信号转导”，“苯丙烷生物合成”和“淀粉和蔗糖代谢”。其中，分别有41、19和26个DEG参与根，茎和下胚轴生长的调控途径。详细而言，下调的IAA17，IAA28，YUCCA9，UPBEAT1和ARR1以及上调的ACR4可能影响根尖分生组织（RAM）中细胞分化与分裂之间的平衡，而下调的CLV1，CRN，AHK4和FAS2可能会干扰茎尖中的干细胞命运分生组织（SAM）。综上所述，目前的研究结果为理解油菜种子发芽期响应涝渍胁迫的幼苗发育的分子机制提供了有价值的信息。