It is a well-known challenge to simulate competition and growth of multiple microorganisms in microbioreactors because of complex interactions among microorganisms, substrate, operational conditions, and structure. In this paper, we developed a multispecies thermal lattice Boltzmann model with the cellular automata model coupled to it to investigate competitive biofilm formation of two aerobic nitrite and ammonium oxidizers in a microbioreactor. Three configurations of the microbioreactor with two heating blocks were simulated to compare the combined effects of the structure and temperature on biofilm growth, detachment, and competition. The results revealed that the heating block temperatures and locations had lower effects on the biofilm growth rate and pattern than the inlet port temperature. Increasing temperature of inlet 2 has more effects on the biofilm growth than increasing temperatures of the two heating blocks. The percentage of the biofilm-occupied grids increases from 7.9 to 12.1% when the inlet temperature at inlet 2 increases from 10 to 50 °C. Two microorganisms showed different rates of responses to changing temperature and structure. The growth of the nitrite oxidizer was about 20% more than that of ammonium oxidizers. This model enables us to understand interactions between individual cells and biofilm communities in the microbioreactor.

中文翻译：

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模拟温度和结构对微生物反应器中多种生物膜竞争和生长的综合影响

在微生物反应器中模拟多种微生物的竞争和生长是众所周知的挑战，因为微生物，底物，操作条件和结构之间存在复杂的相互作用。在本文中，我们开发了一种带有细胞自动机模型的多物种热晶格Boltzmann模型，以研究微生物反应器中两种有氧亚硝酸盐和铵氧化剂的竞争性生物膜形成。模拟了具有两个加热块的微生物反应器的三种配置，以比较结构和温度对生物膜生长，分离和竞争的综合影响。结果表明，加热块的温度和位置对生物膜生长速率和模式的影响比入口温度低。入口2的温度升高对生物膜生长的影响大于两个加热块的温度升高。当入口2的入口温度从10°C上升到50°C时，生物膜占据的栅格的百分比从7.9％上升到12.1％。两种微生物对温度和结构变化的反应速率不同。亚硝酸盐氧化剂的生长量比铵氧化剂的生长量高约20％。该模型使我们能够了解单个细胞与微生物反应器中生物膜群落之间的相互作用。两种微生物对温度和结构变化的反应速率不同。亚硝酸盐氧化剂的生长量比铵氧化剂的生长量高约20％。该模型使我们能够了解单个细胞与微生物反应器中生物膜群落之间的相互作用。两种微生物对温度和结构变化的反应速率不同。亚硝酸盐氧化剂的生长量比铵氧化剂的生长量高约20％。该模型使我们能够了解单个细胞与微生物反应器中生物膜群落之间的相互作用。