ABSTRACT

SERINC5 is a multi-pass transmembrane protein that is thought to play a role in serine incorporation during cellular membrane biosynthesis. This protein has also been identified as a human immunodeficiency virus Type 1 (HIV-1) restriction factor. The paucity of monoclonal antibodies (mAbs) against SERINC5 has posed a challenge for the study of the endogenous protein. Here we report the development of novel anti-SERINC5 mAbs that target three distinct loops on the protein. We demonstrate that these SERINC5 mAbs can be used to detect endogenously expressed SERINC5 protein in various cell lines using Western blot, whole-cell ELISA, flow cytometry, and immunocytochemistry. We further show that some of these antibodies can detect SERINC5 that is present in HIV-1 viral stocks. These antibodies will aid in the characterization of the functions and mechanisms of action of SERINC5 in different cell types.

摘要

SERINC5是一种多通道跨膜蛋白，被认为在细胞膜生物合成过程中在丝氨酸掺入中发挥作用。该蛋白还被鉴定为人类1型免疫缺陷病毒（HIV-1）限制因子。缺乏针对SERINC5的单克隆抗体（mAbs）对内源蛋白质的研究提出了挑战。在这里，我们报告了针对该蛋白质上三个不同环的新型抗SERINC5 mAb的开发。我们证明，这些SERINC5 mAb可用于使用Western blot，全细胞ELISA，流式细胞仪和免疫细胞化学检测各种细胞系中内源表达的SERINC5蛋白。我们进一步表明，这些抗体中的一些可以检测HIV-1病毒原种中存在的SERINC5。