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Introduction of a thrombin sensor based on its interaction with dabigatran as an oral direct thrombin inhibitor
Biomaterials Advances ( IF 7.9 ) Pub Date : 2020-08-22 , DOI: 10.1016/j.msec.2020.111417
Giti Paimard , Mohammad Bagher Gholivand , Mojtaba Shamsipur , Elahe Ahmadi , Mohsen Shahlaei

Dabigatran (DAB) is a direct thrombin inhibitor used for preventing blood clots and emboli after orthopedic surgery. The DAB - thrombin interaction was followed by fluorescence and UV–Vis spectroscopic methods. The binding of DAB to thrombin was also modeled by the molecular docking method. The obtained experimental results were consistent with theoretical results. The voltammetric method was also tested for DAB - thrombin interaction. Based on voltammetric findings, carbon paste electrode containing graphite powder, paraffin oil, MWCNTs, and DAB was constructed and used for thrombin monitoring after investigation of the DAB oxidation mechanism for the first time. The decrease in the linear sweep voltammetric (LSV) peak current of DAB in the presence of thrombin was utilized for the thrombin analysis. The calibration plot was linear in the concentration range of 1 to 70 nM (R2 = 0.9992) by LSV technique with a detection limit of 0.3 nM. The applicability of the proposed sensor was evaluated by the determination of thrombin in human serum as a real sample.



中文翻译:

基于与达比加群相互作用作为口服直接凝血酶抑制剂的凝血酶传感器的推出

达比加群(DAB)是一种直接的凝血酶抑制剂,用于预防骨科手术后的血块和栓子。DAB-凝血酶相互作用之后是荧光和UV-Vis光谱法。DAB与凝血酶的结合也通过分子对接方法进行建模。实验结果与理论结果相吻合。伏安法还测试了DAB-凝血酶的相互作用。根据伏安法的发现,在首次研究DAB氧化机理后,构建了包含石墨粉,石蜡油,MWCNT和DAB的碳糊电极,并将其用于凝血酶监测。在存在凝血酶的情况下,DAB的线性扫描伏安(LSV)峰值电流的降低用于凝血酶分析。 通过LSV技术的R 2 = 0.9992),检出限为0.3 nM。拟议传感器的适用性通过测定人血清中的真实样品中的凝血酶来评估。

更新日期:2020-09-11
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