In this work, supercritical fluid extraction (SFE) was employed to fully utilize patchouli plant including the stems to extract patchouli oil. In the conventional extraction by steam distillation, patchouli stems are often underutilized and discarded as waste. Using Taguchi’s orthogonal array method, the effect of pressure (10–20 MPa), temperature (35–45 °C) and CO₂ flowrate (40–80 g/min) on SFE yield, antioxidant activity and patchoulol concentration from patchouli plant were evaluated. The SFE process conditions were optimized based on the selective yield of patchoulol as patchoulol is generally considered as an indicator of the patchouli oil quality. SFE with the optimized pressure of 15 MPa, temperature of 45 °C and CO₂ flow rate of 60 g/min obtained yield, antioxidant activity and patchoulol concentration of 3.51 %, IC₅₀:1.21 mg/mL and 0.2953 g patchoulol/g oil respectively. The results were compared with steam distillation where SFE shown significantly improved yield and antioxidant activity over the distillation process.

在这项工作中，采用超临界流体萃取（SFE）来充分利用广patch香植物（包括茎）来提取广patch香油。在通过蒸汽蒸馏的常规提取中，广patch香茎经常未被充分利用，并作为废物丢弃。使用田口的正交阵列法，压力（10–20 MPa），温度（35–45°C）和CO ₂流量（40–80 g / min）对广patch香植物的SFE产量，抗氧化活性和广patch香酚浓度的影响评估。SFE的工艺条件基于广patch香酚的选择性收率进行了优化，因为广patch香酚通常被认为是广oul香油质量的指标。SFE的最佳压力为15 MPa，温度为45°C和CO ₂流速为60 g / min，产量，抗氧化活性和广patch香酚浓度为3.51％，IC _50为：1.21 mg / mL和0.2953 g广patch香酚/ g油。将结果与蒸汽蒸馏进行了比较，在蒸汽蒸馏中，SFE显示出比蒸馏过程明显提高的收率和抗氧化活性。