Aspergillus flavus is the predominant species that produce aflatoxins in stored peanuts under favourable conditions. This study aimed to describe the growth and aflatoxin production by two A. flavus strains isolated from imported raw peanuts and to model the effects of temperature and a_w on their colony growth rate as a function of temperature and a_w in Peanut Meal Extract Agar (PMEA). A full factorial design with seven a_w levels (0.85–0.98 a_w) and five temperature levels (20–40 °C) was used to investigate the growth and aflatoxin production. Colony diameter was measured daily for 28 days while AFB₁ and total aflatoxin were determined on day 3, 7, 14, and 21. The maximum colony growth rate, μ_max (mm/day) was estimated by using the primary model of Baranyi, and the μ_max was then fitted to the secondary model; second-order polynomial and linear Arrhenius-Davey to describe the colony growth rate as a function of temperature and a_w. The results indicated that both strains failed to grow at temperature of 20 °C with a_w <0.94 and a_w of 0.85 for all temperatures except 30 °C. The highest growth rate was observed at 30 °C, with 0.98 a_w for both strains. The analysis of variance showed a significant effect of strain, temperature, and a_w on the fungal growth and aflatoxin production (p < 0.05). Furthermore, both secondary models were in good agreement with the observed μ_max. However, the polynomial model was found to be a better predictor of the experimental data. A similar pattern was observed in aflatoxin production but in a narrower range of temperature (25–35 °C) and a_w (0.92–0.98 a_w). The highest production of aflatoxins was observed on day 21 at 30 °C with the a_w level of 0.98 for both strains. Overall, the current findings may help in improving the mycotoxin management and intervention strategies in peanuts, especially during storage.

黄曲霉菌是在有利条件下在储存的花生中产生黄曲霉毒素的主要物种。本研究旨在描述由两个生长和黄曲霉毒素生产黄曲霉菌株从进口原料花生分离和温度的影响和模型_瓦特在其菌落生长速率作为温度的函数和_瓦特在花生饼粉提取物琼脂（ PMEA）。全因子设计具有七个一_瓦特水平（0.85-0.98一个_瓦特使用）和五个温度水平（20-40℃）进行调查的生长和黄曲霉毒素生产。每天测量28天的菌落直径，同时使用AFB ₁和总黄曲霉毒素进行第3天，7天，14确定的，和21的最大集落生长率，μ_最大（毫米/天）通过使用Baranyi的主模型估计，并且μ_最大然后装配到次级模型; 二阶多项式和线性Arrhenius-Davey来描述菌落的生长速率与温度和_w的关系。结果表明，两种菌株均未能在20°C的温度下生长，_w＜0.94，且除30°C以外的所有温度的_w均为0.85。在30°C时观察到最高的生长速率，两种菌株的生长速率均为0.98 a _w。方差分析表明应变，温度的显著效果，和_瓦特对真菌生长和黄曲霉毒素产量的影响（p  <0.05）。此外，两个次要模型都与观察到的μmax_高度吻合。但是，发现多项式模型可以更好地预测实验数据。在黄曲霉毒素的生产中观察到了类似的模式，但是在较窄的温​​度范围（25–35°C）和_w（0.92–0.98 a _w）下。黄曲霉毒素的最高产量在第21天在30°C时观察到，两种菌株的_w水平均为0.98。总体而言，当前的发现可能有助于改善花生中的霉菌毒素管理和干预策略，尤其是在储存期间。