The nature and features of organization of reserve polysaccharides in three species of the genus Pelomyxa—P. palustris, P. belevskii, and P. stagnalis—were studied using light and transmission electron microscopy. We applied the periodic acid-Schiff reaction that is a highly selective method for detecting glycogen. The fluorescent dye auramine-SO2 (Au-SO2) was used as a Schiff-type reagent. The densely packed aggregates of glycogen that form the morphologically differentiated organelle-like bodies are revealed in the cytoplasm in all studied species. The organization of these bodies is characterized by the species-specific features, while in most cases, their size and number in the cells vary depending on the season of the year. Although in all the cases we studied, these bodies do not have their own boundary membrane, in fact, they are surrounded by membranous structures. These structures differ in a variety of Pelomyxa species. We concluded that there are two groups of species in the genus Pelomyxa. The first one includes organisms containing glycogen structures in the cytoplasm (P. palustris, P. belevskii, P. stagnalis, P. binucleata, P. corona, P. secunda). No inclusions resembling glycogen bodies were found in P. flava, P. paradoxa, P. gruberi, and P. prima that form the second group.

中文翻译：

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三种 Pelomyxa 物种（古变形虫、Pelobiontida）中储备多糖的组织性质和特征

Pelomyxa-P. 三个物种中储备多糖的组织性质和特征。palustris、P. belevskii 和 P. stagnalis——使用光学和透射电子显微镜进行了研究。我们应用了高碘酸-希夫反应，这是一种检测糖原的高选择性方法。荧光染料金胺-SO2 (Au-SO2) 用作希夫型试剂。在所有研究物种的细胞质中都显示出密集的糖原聚集体，形成形态上分化的细胞器样小体。这些身体的组织以物种特异性特征为特征，而在大多数情况下，它们在细胞中的大小和数量因一年中的季节而异。尽管在我们研究的所有案例中，这些物体都没有自己的边界膜，事实上，它们被膜结构包围。这些结构在各种 Pelomyxa 物种中有所不同。我们得出结论，Pelomyxa 属中有两组物种。第一个包括在细胞质中含有糖原结构的生物（沼泽对虾、贝列夫斯基对虾、stagnalis、双核对虾、电晕对虾、二核对虾）。在构成第二组的 P. flava、P. paradoxa、P. gruberi 和 P. prima 中没有发现类似糖原体的内含物。