Understanding the complexity and biodiversity of fungal communities associated with the wheat endosphere can facilitate the identification of novel strains that might be beneficial to the host plant. However, the differentiation and taxonomic classification of the endosphere-associated fungi with respect to various cultivars and plant organs are challenging, time-consuming, and expensive, even with the use of molecular techniques. In the present work, we describe a fast, simple, and low-cost method based on high-resolution melting PCR (HRM-PCR) for the identification and differentiation of wheat endogenous fungal isolates. Using this approach, we differentiated 28 fungal isolates, which belonged to five different genera, namely Alternaria, Penicillium, Epicoccum, Fusarium, and Trichoderma. Furthermore, the results of the study revealed that this method can allow large-scale screening of cultured samples.

了解与小麦内圈相关的真菌群落的复杂性和生物多样性可以促进鉴定可能对宿主植物有益的新菌株。然而，即使使用分子技术，与各种栽培品种和植物器官有关的内球相关真菌的分化和分类学分类也是具有挑战性，耗时且昂贵的。在当前的工作中，我们描述了一种基于高分辨率熔解PCR（HRM-PCR）的快速，简单和低成本的方法，用于鉴定和区分小麦内源真菌分离株。使用这种方法，我们区分了28个真菌分离株，它们属于五个不同的属，即链格孢属，青霉属，表球菌属。，镰刀菌和木霉。此外，研究结果表明，该方法可以大规模筛选培养样品。