DNA extraction can be lengthy and sometimes ends up with amplification inhibitors. We present the potential of recombinase polymerase amplification (RPA) to replace plant DNA extraction. In our rapid 'RPA-PCR couple' concept, RPA is tuned to slower reaction kinetics to promote amplification of long targets. RPA primers amplify target and some flanking regions directly from simple plant macerates. Then PCR primers exponentially amplify the target directly from the RPA reaction. We present the coupling of RPA with conventional, TaqMan and SYBR Green PCR assays. We applied the concept to strawberry Phytophthora pathogens and the Phytophthora identification marker atp9-nad9. We found RPA-PCR couple specific, sensitive and reliable. The approach may also benefit other difficult samples such as food, feces and ancient samples.

中文翻译：

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RPA-PCR 对：一种加快植物诊断和克服 PCR 抑制剂的方法。

DNA 提取可能很长，有时最终会出现扩增抑制剂。我们展示了重组酶聚合酶扩增 (RPA) 替代植物 DNA 提取的潜力。在我们的快速“RPA-PCR 对”概念中，RPA 被调整为较慢的反应动力学，以促进长目标的扩增。RPA 引物直接从简单的植物浸渍液中扩增目标区域和一些侧翼区域。然后 PCR 引物直接从 RPA 反应中以指数方式扩增目标。我们介绍了 RPA 与传统、TaqMan 和 SYBR Green PCR 检测的耦合。我们将该概念应用于草莓疫霉属病原体和疫霉属鉴定标记atp9-nad9. 我们发现 RPA-PCR 对是特异性的、灵敏的和可靠的。该方法还可能有益于其他困难样品，例如食物、粪便和古代样品。