Grave's disease (GD) occurs due to an autoimmune dysfunction of thyroid gland cells, leading to manifestations consistent with hyperthyroidism. Various studies have confirmed the link between autoimmune conditions and changes in the composition of intestinal microbial organisms. However, few studies have assessed the relationship between the disease of GD and the changes of intestinal microbiota. Therefore, this study aimed to investigate changes in intestinal flora that may occur in the setting of GD. Thirty-nine patients with GD and 17 healthy controls were enrolled for fecal sample collection. 16S rRNA sequencing was used to analysis the diversity and composition of the intestinal microbiota. High-throughput sequencing of 16S rRNA genes of intestinal flora was performed on Illumina Hiseq2500 platform. Comparing to healthy individuals, the number of Bacilli, Lactobacillales, Prevotella, Megamonas and Veillonella strains were increased, whereas the number of Ruminococcus, Rikenellaceae and Alistipes strains were decreased among patients with GD. Furthermore, patients with GD showed a decrease in intestinal microbial diversity. Therefore, it indicates that the diversity of microbial strains is significantly deduced in GD patients, and patients with GD will undergo significant changes in intestinal microbiota, by comparing the intestinal flora of GD and healthy controls. Theses conclusion are expected to provide a preliminary reference for further researches on the interaction mechanism between intestinal flora and GD.

中文翻译：

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格雷夫斯病中肠道菌群的变化：一项前瞻性临床研究。

严重疾病（GD）的发生是由于甲状腺细胞的自身免疫功能异常，导致与甲状腺功能亢进相一致的表现。各种研究已证实自身免疫状况与肠道微生物组成变化之间的联系。但是，很少有研究评估GD疾病与肠道菌群变化之间的关系。因此，本研究旨在调查在GD环境中可能发生的肠道菌群变化。收集了39例GD患者和17名健康对照者的粪便样本。16S rRNA测序用于分析肠道菌群的多样性和组成。在Illumina Hiseq2500平台上进行了肠道菌群16S rRNA基因的高通量测序。与健康的人相比，GD患者中，芽孢杆菌，乳杆菌，普雷沃氏菌，大摩纳哥和韦永氏菌的数量增加，而Ruminococcus，Rikenellaceae和Alistipes菌株的数量减少。此外，GD患者显示肠道微生物多样性降低。因此，这表明通过比较GD的肠道菌群和健康对照，GD患者中微生物菌株的多样性被显着降低，GD患者的肠道菌群将发生显着变化。这些结论有望为进一步研究肠道菌群与GD之间的相互作用机理提供初步参考。GD患者中Renkenellaceae和Alistipes菌株减少。此外，GD患者显示肠道微生物多样性降低。因此，这表明通过比较GD的肠道菌群和健康对照，GD患者中微生物菌株的多样性被显着降低，GD患者的肠道菌群将发生显着变化。这些结论有望为进一步研究肠道菌群与GD之间的相互作用机理提供初步参考。GD患者中Renkenellaceae和Alistipes菌株减少。此外，GD患者显示肠道微生物多样性降低。因此，这表明通过比较GD的肠道菌群和健康对照，GD患者中微生物菌株的多样性被显着降低，GD患者的肠道菌群将发生显着变化。这些结论有望为进一步研究肠道菌群与GD之间的相互作用机理提供初步参考。通过比较GD和健康对照组的肠道菌群，GD患者的肠道菌群将发生显着变化。这些结论有望为进一步研究肠道菌群与GD之间的相互作用机理提供初步参考。通过比较GD和健康对照组的肠道菌群，GD患者的肠道菌群将发生显着变化。这些结论有望为进一步研究肠道菌群与GD之间的相互作用机理提供初步参考。