Haskap (Lonicera caerulea subsp. edulis) is a deciduous shrub that produces blue-black edible berries with a sour-sweet taste. By expanding fruit color variation, the value of agricultural products is enhanced. Interspecific hybrids were obtained from crossings between Haskap and red-fruit bearing Miyama-uguisukagura (Lonicera gracilipes). The fruit color of the interspecific hybrids obtained was red-purple. Fruit color in Haskap is mainly affected by the concentration of anthocyanin. However, there are no reports on the chemical determinants of fruit color in interspecific hybrids between Haskap and Miyama-uguisukagura. We evaluated anthocyanin components in these hybrids and their parents using liquid chromatography/tandem mass spectrometry, and revealed the presence of five different kinds of anthocyanins. The major anthocyanin in interspecific hybrids and Haskap was cyanidin 3-glucoside, while in Miyama-uguisukagura, it was cyanidin 3,5-diglucoside. Some genotypes among interspecific hybrids showed higher concentrations of cyanidin 3,5-diglucoside and peonidin 3,5-diglucoside, compared with their parents. The genotypes of interspecific hybrids and the parents were evaluated by principal component analysis of anthocyanin concentration. Our study contributes to the identification of anthocyanin composition in fruits of interspecific hybrids and in expanding fruit color variation when breeding new varieties.

Haskap（蓝靛亚种果）是一种落叶灌木产生蓝黑色浆果食用带有酸甜的味道。通过扩大水果的颜色变化，增加了农产品的价值。种间杂种是从Haskap和带有红果的Miyama-uguisukagura（忍冬（Lonicera gracilipes））。获得的种间杂种的果实颜色为红紫色。Haskap中的果实颜色主要受花青素浓度的影响。但是，尚无关于Haskap和Miyama-uguisukagura之间种间杂种中果实颜色的化学决定因素的报道。我们使用液相色谱/串联质谱法评估了这些杂种及其亲本中的花色苷成分，并揭示了五种花色苷的存在。种间杂种和Haskap中主要的花色苷是花青素3-葡糖苷，而在Miyama-uguisukagura中，花色苷是3,5-二葡糖苷。种间杂种中的某些基因型显示，与其亲本相比，花青素3,5-二葡萄糖苷和peonidin 3,5-二葡萄糖苷的浓度更高。种间杂种和亲本的基因型通过花色苷浓度的主成分分析进行评估。我们的研究有助于鉴定种间杂种果实中的花色苷成分，并有助于在育种新品种时扩大果实的颜色变化。