Osun-Osogbo Grove has a long history of healing and therapeutic claims by adherent believers, in spite of advancement in medicine. Scientists made attempts at investigating the biodiversity of the Grove, till date, there has not been convergence point between science and these indigenous beliefs. This study identified the presence of therapeutic agents in the water of Osun-Osogbo River, paying attention to at least six parameters; vitamin, phosphate, nitrate, amino acid, hormone and trace metal. Water samples were taken from two different sites during pre, during and post raining sessions (April 2017 - September 2019) were analysed using High Performance Liquid Chromatography (HPLC), Gas Chromatography Mass Spectroscopy (GC-MS) and Atomic Absorption Spectrometer (AAS). Trace metal analysis revealed an average of 0.009-0.079 mg/Kg Zinc from site one and lower in site two. The mean value of manganese at both sites was virtually the same at 0.018-0.313 mg/kg, aluminum content was 0.045-0.179mg/Kg at site one, 0.050-0.192mg/kg at site two, cobalt was 0.024 mg/kg at site one, 0.026 mg/kg at site two while nickel was 0.006 mg/kg and 0.004 mg/kg for site one and two respectively. HPLC analysis shows mean Methionine content at both sites is higher than the FDA (56.6 ug/mL); site one had 74.41 ug/mL while site two had 57.11 ug/mL The mean values of two water-soluble vitamins; Thiamine (B1) was 3.758 mg/Kg and 2.355 mg/Kg while Pyridoxine (B6) was 0.108 mg/Kg and 0.072 mg/Kg at site one and two. GCMS analysis of steroidal content revealed values below lowest observed effect level (LOEL), testosterone (4.8 ng/L) and estrogen (2.4 ng/L) were still elevated while ethinylestradiol and estriol were ≥1.5 ng/L. Summarily, site one the major part for spiritual activities showed higher essential nutrient contents than site two which support the enrichment and potential therapeutic properties of the Osun river water. However, further scientific research is required to ensure that these therapeutic potentials supersede the toxicological effect.

中文翻译：

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营养物质：Osun河的微量金属，微量营养素，雌激素和B-维生素含量：从尼日利亚西南部进入几内亚大西洋湾的一条河

尽管医学进步，Osun-Osogbo Grove仍拥有悠久的历史，受到信徒们的治愈和治疗要求。科学家们试图研究格罗夫的生物多样性，直到今天，科学与这些土著信仰之间还没有一个融合点。这项研究确定了Osun-Osogbo河水中存在治疗剂，并注意至少六个参数。维生素，磷酸盐，硝酸盐，氨基酸，激素和微量金属。使用高效液相色谱（HPLC），气相色谱质谱（GC-MS）和原子吸收光谱仪（AAS）在降雨前后，2017年4月至2019年9月期间从两个不同的地点采集水样进行分析。痕量金属分析显示平均值为0.009-0。第一站点的锌含量为079 mg / Kg，第二站点的锌含量较低。两个位点的锰平均值几乎相同，为0.018-0.313 mg / kg，第一个位点的铝含量为0.045-0.179mg / Kg，第二个位点的铝含量为0.050-0.192mg / kg，第二个位点的钴为0.024 mg / kg第一站点，第二站点为0.026 mg / kg，而第一站点和第二站点的镍分别为0.006 mg / kg和0.004 mg / kg。HPLC分析显示，两个位点的平均蛋氨酸含量均高于FDA（56.6 ug / mL）。第一位为74.41 ug / mL，第二位为57.11 ug / mL两种水溶性维生素的平均值。在第一个和第二个部位的硫胺素（B1）分别为3.758 mg / Kg和2.355 mg / Kg，而吡ox醇（B6）分别为0.108 mg / Kg和0.072 mg / Kg。GCMS对甾体含量的分析显示，其值低于最低观察到的作用水平（LOEL），睾丸激素（4.8 ng / L）和雌激素（2。4 ng / L）仍升高，而炔雌醇和雌三醇≥1.5 ng / L。综上所述，地点一是精神活动的主要部分，其营养素含量高于地点二，这支持了乌孙河水的富集和潜在的治疗特性。但是，需要进一步的科学研究来确保这些治疗潜力取代毒理学作用。