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Bacteroides fragilis fucosidases facilitate growth and invasion of Campylobacter jejuni in the presence of mucins.
Cellular Microbiology ( IF 3.4 ) Pub Date : 2020-08-22 , DOI: 10.1111/cmi.13252
Yvette M C A Luijkx 1, 2 , Nancy M C Bleumink 2 , Jianbing Jiang 3, 4 , Herman S Overkleeft 3 , Marc M S M Wösten 2 , Karin Strijbis 2 , Tom Wennekes 1
Affiliation  

The enteropathogenic bacterium, Campylobacter jejuni, was considered to be non‐saccharolytic, but recently it emerged that l‐fucose plays a central role in C. jejuni virulence. Half of C. jejuni clinical isolates possess an operon for l‐fucose utilisation. In the intestinal tract, l‐fucose is abundantly available in mucin O‐linked glycan structures, but C. jejuni lacks a fucosidase enzyme essential to release the l‐fucose. We set out to determine how C. jejuni can gain access to these intestinal l‐fucosides. Growth of the fuc + C. jejuni strains, 129,108 and NCTC 11168, increased in the presence of l‐fucose while fucose permease knockout strains did not benefit from additional l‐fucose. With fucosidase assays and an activity‐based probe, we confirmed that Bacteriodes fragilis, an abundant member of the intestinal microbiota, secretes active fucosidases. In the presence of mucins, C. jejuni was dependent on B. fragilis fucosidase activity for increased growth. Campylobacter jejuni invaded Caco‐2 intestinal cells that express complex O‐linked glycan structures that contain l‐fucose. In infection experiments, C. jejuni was more invasive in the presence of B. fragilis and this increase is due to fucosidase activity. We conclude that C. jejuni fuc + strains are dependent on exogenous fucosidases for increased growth and invasion.

中文翻译:

在粘蛋白存在的情况下,脆弱拟杆菌岩藻糖苷酶促进空肠弯曲杆菌的生长和入侵。

肠道致病菌空肠弯曲杆菌被认为是非糖解菌,但最近发现l-岩藻糖在空肠弯曲杆菌毒力中起着核心作用。一半的空肠弯曲杆菌临床分离株具有用于l-岩藻糖利用的操纵子。在肠道中,-岩藻糖是可大量获得的粘蛋白ø -连接的聚糖结构,但空肠弯曲杆菌缺少岩藻糖苷酶必需以释放岩藻糖。我们着手确定空肠弯曲杆菌如何获得这些肠道l-岩藻糖苷。fuc的成长 + C. jejuni 菌株 129,108 和 NCTC 11168 在l-岩藻糖存在下增加,而岩藻糖通透酶敲除菌株没有从额外的l-岩藻糖中获益。通过岩藻糖苷酶测定和基于活性的探针,我们证实脆弱拟杆菌是肠道微生物群的丰富成员,分泌活性岩藻糖苷酶。在粘蛋白存在的情况下,空肠弯曲杆菌依赖脆弱拟杆菌岩藻糖苷酶活性来促进生长。空肠弯曲杆菌侵入 Caco-2 肠道细胞,该细胞表达含有l-岩藻糖的复杂O-连接聚糖结构。在感染实验中,C. jejuni脆弱拟杆菌存在下更具侵入性,这种增加是由于岩藻糖苷酶活性。我们得出结论,C. jejuni fuc  + 菌株依赖外源岩藻糖苷酶来增加生长和入侵。
更新日期:2020-08-22
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