Insect Biochemistry and Molecular Biology ( IF 3.8 ) Pub Date : 2020-08-22 , DOI: 10.1016/j.ibmb.2020.103451 Yuzhen Lu 1 , Fanghua Su 1 , Kesen Zhu 1 , Mengyao Zhu 1 , Qilin Li 1 , Qihao Hu 1 , Jie Zhang 1 , Ruonan Zhang 1 , Xiao-Qiang Yu 2
C-type lectins (CTLs) recognize various glycoconjugates through carbohydrate recognition domains (CRDs) and they play important roles in immune responses. In this study, comparative genomic analysis of CTLs were performed in 7 holometabolous species. CTL-S1 to S8 and CTL-X1 to X4 orthologous groups existed in the 7 species, while CTL-X5 group with dual-CRD, CTL-S11 group with triple-CRD, CTL-S9 group with a long C-terminus and Lepidopteran specific CTL-S10 group were not conserved. SliCTL-S12 to S14 cluster was only present in Spodoptera litura, and CTL-S genes were expanded on chromosomes 2 L and 2 R in Drosophila melanogaster. Most IMLs were clustered into three groups and the numbers of IMLs vary among species due to gene duplications. D. melanogaster specific CTLs and Lepidopteran IMLs within each of the three groups evolved more rapidly with higher dN/dS ratios. Two CRDs in IMLs clustered into two clades, with conserved Cys4-Cys5 and Cys1-Cys2 bonds in the first and second CRDs, respectively. The CTL-S and CTL-X family members in S. litura were mainly expressed in the fat body of 5th but not 6th instar larvae, and responded differently to S. litura nucleopolyhedrovirus (SpltNPV) and Nomuraea rileyi infection. The transcription levels of SliCTLs that expressed in fat body but not highly expressed in hemocytes were decreased at the middle and late stages of SpltNPV infection, and the mRNA levels of SliCTLs highly or specifically expressed in hemocytes were mainly decreased by SpltlNPV, N. rileyi and Bacillus thuringiensis infection. These results provide valuable information for further exploration of CTL functions in host-pathogen interaction.
中文翻译:
七个全代谢昆虫物种C型凝集素结构域基因的比较基因组分析。
C型凝集素(CTL)通过碳水化合物识别域(CRD)识别各种糖缀合物,它们在免疫反应中起重要作用。在这项研究中,对7种全代谢物种进行了CTL的比较基因组分析。CTL-S1至S8和CTL-X1至X4直系同源物种存在于这7个物种中,而CTL-X5组具有双CRD,CTL-S11组具有三重CRD,CTL-S9组具有长C末端和鳞翅目。特定的CTL-S10组不保守。SliCTL-S12至S14簇仅存在于斜纹夜蛾中,而CTL-S基因在果蝇的2 L和2 R染色体上扩增。大多数IML分为三类,由于基因重复,物种间的IML数量也有所不同。黑腹果蝇 三组中每组的特定CTL和鳞翅目IML随着dN / dS比的提高而发展得更快。IML中的两个CRD聚集成两个进化枝,在第一和第二CRD中分别具有保守的Cys4-Cys5和Cys1-Cys2键。斜纹夜蛾的CTL-S和CTL-X家族成员主要在第5龄幼虫的脂肪体中表达,而在第6龄幼虫的脂肪体中不表达,并且对斜纹夜蛾核多角体病毒(SpltNPV)和野村奈莫氏菌感染的反应不同。在 SpltNPV感染的中后期,在脂肪体内表达但在血细胞中表达不高的SliCTL的转录水平 降低,并且SliCTL的mRNA水平下降在血细胞高度或特异性表达的主要减少SpltlNPV,N. rileyi和苏云金芽孢杆菌的感染。这些结果为进一步探讨宿主-病原体相互作用中CTL功能提供了有价值的信息。