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In vitro evaluation of potential benefits of a silica-rich thermal water (Monfortinho Thermal Water) in hyperkeratotic skin conditions
International Journal of Biometeorology ( IF 3.2 ) Pub Date : 2020-08-21 , DOI: 10.1007/s00484-020-01986-x
Ana Sofia Oliveira 1 , Cátia Vicente Vaz 1 , Ana Silva 2 , Sara Correia 1 , Raquel Ferreira 1, 3 , Luiza Breitenfeld 1, 3 , José Martinez-de-Oliveira 1, 3 , Rita Palmeira-de-Oliveira 1, 2, 4 , Cláudia Pereira 2, 5 , Maria Teresa Cruz 2, 6 , Ana Palmeira-de-Oliveira 1, 3, 4
Affiliation  

Thermal therapy has gained popularity over the years, and Portugal is one of the richest European countries in mineral therapeutic waters. The interest in the use of these natural mineral waters (NMW) for dermatologic purposes is continuously growing but there is a lack of scientific studies supporting its health benefits. The study aims to investigate the effect of a silica-rich NMW in skin cell homeostasis using two representative cell lines of the epidermis and dermis, keratinocytes and fibroblasts, respectively, in addition to a macrophage cell line. Mouse skin fibroblasts, macrophages and human keratinocytes were exposed to culture medium prepared with NMW. Cell metabolism (MTT or resazurin assays) and cell proliferation (trypan blue exclusion dye assay) were investigated. Migration (scratch-wound assay) and senescence (β-galactosidase activity assay) of fibroblasts were also studied. Exposure to NMW compromised the cell metabolic state of all the cell lines tested. This impairment was more pronounced in skin keratinocytes (60% reduction) relatively to skin fibroblasts (45% reduction) or macrophages (25% reduction). Proliferation of macrophages was reduced threefold upon exposure to thermal water, compared to controls. No differences were observed in migration between fibroblasts exposed to NMW and controls, while a potentiation of senescence of these cells was observed. Our results shed light in the bioactive effects of a silica-rich NMW supporting its therapeutic use. A reduction in both cell metabolism and proliferation of keratinocytes and macrophages supports the empirical clinical benefits of this NMW in hyperkeratotic conditions, such as psoriasis and atopic dermatitis.

中文翻译:

富含二氧化硅的温泉水(Monfortinho 温泉水)对角化过度皮肤病的潜在益处的体外评估

多年来,热疗越来越受欢迎,葡萄牙是欧洲矿泉水最丰富的国家之一。将这些天然矿泉水 (NMW) 用于皮肤病学的兴趣不断增长,但缺乏支持其健康益处的科学研究。该研究旨在研究富含二氧化硅的 NMW 对皮肤细胞稳态的影响,除了巨噬细胞系外,还分别使用表皮和真皮、角质形成细胞和成纤维细胞的两种代表性细胞系。小鼠皮肤成纤维细胞、巨噬细胞和人角质形成细胞暴露于用 NMW 制备的培养基中。研究了细胞代谢(MTT 或刃天青测定)和细胞增殖(台盼蓝排斥染料测定)。还研究了成纤维细胞的迁移(划痕试验)和衰老(β-半乳糖苷酶活性试验)。暴露于 NMW 会损害所有测试细胞系的细胞代谢状态。相对于皮肤成纤维细胞(减少 45%)或巨噬细胞(减少 25%),这种损伤在皮肤角质形成细胞(减少 60%)中更为明显。与对照组相比,暴露于热水中后巨噬细胞的增殖减少了三倍。未观察到暴露于 NMW 的成纤维细胞和对照之间的迁移差异,同时观察到这些细胞的衰老增强。我们的结果阐明了支持其治疗用途的富含二氧化硅的 NMW 的生物活性作用。
更新日期:2020-08-21
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