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Effect of pH on xylitol production by Candida species from a prairie cordgrass hydrolysate
Zeitschrift für Naturforschung C ( IF 2 ) Pub Date : 2020-11-26 , DOI: 10.1515/znc-2020-0140
Samatha S R Rudrangi 1 , Thomas P West 1
Affiliation  

Abstract Using hydrolysates of the North American prairie grass prairie cordgrass buffered at pH 4.5, 5.0, 5.5 or 6.0, xylitol production, xylitol yield, cell biomass production and productivity were investigated for three strains of yeast Candida. Of the three strains, the highest xylitol concentration of 20.19 g xylitol (g xylose consumed)−1 and yield of 0.89 g xylitol (g xylose consumed)−1 were produced by Candida mogi ATCC 18364 when grown for 120 h at 30° C on the pH 5.5-buffered hydrolysate-containing medium. The highest biomass level being 7.7 g cells (kg biomass)−1 was observed to be synthesized by Candida guilliermondii ATCC 201935 after 120 h of growth at 30° C on a pH 5.5-buffered hydrolysate-containing medium. The highest xylitol specific productivity of 0.73 g xylitol (g cells h)−1 was determined for C. guilliermondii ATCC 20216 after 120 h of growth at 30°C on a pH 5.0-buffered hydrolysate-containing medium. Xylitol production and yield by the three Candida strains was higher on prairie cordgrass than what was previously observed for the same strains after 120 h at 30° C when another North American prairie grass big bluestem served as the plant biomass hydrolysate indicating that prairie cordgrass may be a superior plant biomass substrate.

中文翻译:

pH 值对来自草原草水解物的念珠菌属生产木糖醇的影响

摘要 使用缓冲在 pH 4.5、5.0、5.5 或 6.0 的北美草原草原草的水解产物,研究了三种酵母念珠菌菌株的木糖醇产量、木糖醇产量、细胞生物量产量和生产力。在三个菌株中,当在 30°C 和pH 5.5 缓冲的含水解产物的培养基。观察到最高生物量水平为 7.7 g 细胞(kg 生物量)-1,由 Candida guilliermondii ATCC 201935 在 30°C 下在 pH 5.5 缓冲的水解产物培养基上生长 120 小时后合成。确定了 0.73 g 木糖醇(g 细胞 h)-1 的最高木糖醇比生产率。guilliermondii ATCC 20216 在 30°C 下在 pH 5.0 缓冲的水解产物培养基上生长 120 小时后。在 30°C 下 120 小时后,当另一种北美草原草大蓝茎作为植物生物量水解物时,草原草本植物的三个念珠菌菌株的木糖醇产量和产量高于先前观察到的相同菌株的木糖醇产量和产量,这表明草原草木可能是优良的植物生物质底物。
更新日期:2020-11-26
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