Cyprinion acinaces are ray-finned fish belongs to genus Cyprinion. Previous studies have reported that this species has two subspecies, Cyprinion acinaces acinaces, and, Cyprinion acinaes hijazi, however, the validity of later was always in doubt. In past, this fishes were classified merely based on morphological characteristics, and modern biotechnology related tools were never used, which would had been helpful, to authenticate the subspecies status between closely related species. This is the first study to report the classification of the major endemic fish species of Saudi Arabia based on phylogenetic analysis. The cytochrome b gene sequences of Cyprinion acinaces acinases, and Carasobarbus aponesis were determined for the first time, and were deposited in public Gene data Bank. The phylogenetic tree clearly grouped the species into two major clusters, which are divided into four sub-clusters. The phylogenetic analysis supports the early taxonomic classification and validated that C. acinaes hijazi is a subspecies of C. acinaces acinaces. The phylogenetic tree constructed from the cytochrome b sequence also indicated that B. aponesis found in Saudi Arabia is genetically closely related to C. luteus.

塞浦路斯pr族属are属鱼类的鳍鳍鱼。先前的研究已报道该物种有两个亚种，Cyprinion acinaces acinaces和Cyrpinion acinaes hijazi，但是，后来的有效性一直令人怀疑。过去，仅根据形态特征对这些鱼进行分类，并且从未使用过与现代生物技术相关的工具，这对确定密切相关物种之间的亚种状态很有帮助。这是第一个基于系统发育分析报告沙特阿拉伯主要特有鱼类物种分类的研究。细胞色素b的基因序列Cyprinion acinaces acinases，和Carasobarbus aponesis首次确定，并保存在公共基因数据库中。系统发育树将物种清楚地分为两个主要簇，分为四个子簇。系统发育分析支持早期的分类学分类，并验证了acinaces hijazi是acinaces acinaces的一个亚种。从细胞色素b序列构建的系统进化树也表明，在沙特阿拉伯发现的B. aponesis与C. luteus在遗传上密切相关。