Two previously undescribed seco-type C₁₉-diterpenoid alkaloids, stylosines A and B (1–2), which possess an unprecedented opened D ring by the C₍₁₅₎–C₍₁₆₎ broken bond forming a five-membered C₍₁₅₎−O–C₍₁₄₎ lactone ring, together with 14 known diterpenoid alkaloids were isolated from the roots of Aconitum stylosum (Ranunculaceae). Compound 1 is the first reported C₁₉-diterpenoid alkaloid with an α-orientation at H-13. Their structures were determined by extensive spectroscopic analyses, NMR calculations and DP4+ analysis. A plausible biosynthetic pathway of the undescribed compounds was proposed. Compounds 1 and 2 showed significant inhibitory activity against the production of inflammatory cytokines (interleukin 1β, cyclooxygenase-2, and tumor necrosis factor α) in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Compounds 1 and 2 exhibited antibacterial activity against Staphylococcus aureus with MIC values of 2.00 and 32.00 μg/mL, respectively.

两个以前未描述开环- C型_19种-diterpenoid生物碱，stylosines A和B（1 - 2），其由C具有前所未有的打开d环_（15） -C _（16）断裂键形成五元C- _{（15 ）} -O-C _（14）内酯环与14种已知的二萜类生物碱是从铁乌头（毛an科）的根中分离出来的。化合物1是第一个报道的具有α的C _19-二萜生物碱-在H-13定向。它们的结构通过广泛的光谱分析，NMR计算和DP4 +分析确定。提出了未描述化合物的可能的生物合成途径。化合物1和2在脂多糖（LPS）诱导的RAW 264.7巨噬细胞中对炎症细胞因子（白介素1β，环氧合酶2和肿瘤坏死因子α）的产生具有显着的抑制活性。化合物1和2表现出抗菌活性对金黄色葡萄球菌为2.00和32.00 MIC值 μ分别克/毫升，。