Research question

To determine the relationship between vitamin D (VitD) status and embryological, clinical pregnancy and live birth outcomes in women undergoing IVF.

Design

Cross-sectional, observational study conducted at a university-affiliated private IVF clinic. A total of 287 women underwent 287 IVF cycles and received a fresh embryo transfer. Patients had their serum 25-hydroxyvitamin D2/D3 (VitD) determined on the day of oocyte retrieval, which was analysed in relation to blastocyst development rate, clinical pregnancy and live birth outcomes.

Results

In stepwise, multivariable logistic regression models, increases in blastocyst development rate, number and quality, along with embryo cryopreservation and utilization rates were associated with women with a sufficient VitD status (≥20 ng/ml). For a single increase in the number of blastocysts generated per cycle or embryos cryopreserved per cycle, the likelihood for the patient to be VitD sufficient was increased by 32% (odds ratio [OR] 1.32, 95% confidence interval [CI] 1.10–1.58, P = 0.002 and OR 1.33, 95% CI 1.10–1.60, P = 0.004, respectively). Clinical pregnancy (40.7% versus 30.8%, P = 0.086) and live birth rates (32.9% versus 25.8%, P = 0.195) in the sufficient VitD group versus the insufficient group were not significantly different and VitD sufficiency was not significantly associated with these outcomes.

Conclusion

A strong relationship was observed between blastocyst development and VitD sufficiency. However, there was no association between VitD and clinical pregnancy or live birth outcomes. Further larger studies are needed to investigate whether the observed effect on blastocyst development may have downstream implications on subsequent clinical pregnancy or live birth rates, and on a potential mechanism where sufficient VitD concentrations are linked to improved IVF outcomes.

中文翻译：

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血清维生素 D 状态与接受 IVF 的女性的囊胚发育率增加有关

研究问题

确定维生素 D (VitD) 状态与接受 IVF 的妇女的胚胎学、临床妊娠和活产结果之间的关系。

设计

在大学附属的私人试管婴儿诊所进行的横断面观察性研究。共有 287 名女性接受了 287 个试管婴儿周期并接受了新鲜胚胎移植。患者在取卵当天测定血清 25-羟基维生素 D2/D3 (VitD)，分析与囊胚发育率、临床妊娠和活产结果的关系。

结果

在逐步的多变量逻辑回归模型中，囊胚发育率、数量和质量以及胚胎冷冻保存和利用率的增加与具有足够 VitD 状态（≥20 ng/ml）的女性相关。对于每个周期产生的囊胚数量或每个周期冷冻保存的胚胎数量的单一增加，患者获得足够 VitD 的可能性增加了 32%（优势比 [OR] 1.32，95% 置信区间 [CI] 1.10–1.58 , P  = 0.002 和 OR 1.33, 95% CI 1.10–1.60, P  = 0.004, 分别）。临床妊娠（40.7% 对 30.8%，P  = 0.086）和活产率（32.9% 对 25.8%，P = 0.195) 在足够的 VitD 组与不足组中没有显着差异，并且 VitD 充足与这些结果没有显着相关性。

结论

在囊胚发育和 VitD 充足性之间观察到很强的关系。然而，VitD 与临床妊娠或活产结果之间没有关联。需要进一步更大规模的研究来调查观察到的对囊胚发育的影响是否可能对随后的临床妊娠或活产率产生下游影响，以及足够的 VitD 浓度与改善 IVF 结果相关的潜在机制。