To improve the developmental potential of in vitro embryos is a long-term concern field for human assisted reproduction and animal in vitro embryo production practice. In the current study, we examined the effects and mechanism of an HDAC6 inhibitor, tubacin, on the maturation of porcine oocytes and in vitro development of porcine IVF embryos. It has been demonstrated the effect of tubacin on the acetylation level of α-tubulin in porcine oocytes.. As a result, the maturation rate of porcine oocytes was significantly improved (P < 0.05), and the following development potent of blastocysts forming rate was also significantly increased (P < 0.05). We found that the increased acetylation of α-tubulin significantly reduced the abnormal rate of microtubule, furthermore, the proportion of mitochondria in the vicinity of in vitro fertilization (IVF) nucleus was significantly enhanced in Metaphase I (MI) and Metaphase II (MII) stages. The expression levels of microtubule assembly genes (TUBA1A, αTAT1 and MAP2) significantly up-regulated in MI and MII stages. Together, these results suggest that treatment of porcine oocytes during maturation with tubacin could promote their IVF embryos developmental competence by altering spindle formation, mitochondrial concentration and genes expression patterns of matured porcine oocytes.

中文翻译：

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通过增加成熟猪卵母细胞乙酰化微管蛋白的 tubacin 处理提高体外受精胚胎的早期发育效力

提高体外胚胎的发育潜力是人类辅助生殖和动物体外胚胎生产实践中长期关注的领域。在目前的研究中，我们研究了 HDAC6 抑制剂 tubacin 对猪卵母细胞成熟和猪体外受精胚胎体外发育的影响和机制。已经证明tubacin对猪卵母细胞α-微管蛋白乙酰化水平的影响。结果，猪卵母细胞的成熟率显着提高（P < 0.05），胚泡形成率的以下发育效力为也显着增加（P < 0.05）。我们发现α-微管蛋白乙酰化的增加显着降低了微管的异常率，此外，体外受精（IVF）核附近的线粒体比例在中期I（MI）和中期II（MII）阶段显着增加。微管组装基因（TUBA1A、αTAT1 和 MAP2）的表达水平在 MI 和 MII 阶段显着上调。总之，这些结果表明，在成熟过程中用 tubacin 处理猪卵母细胞可以通过改变成熟猪卵母细胞的纺锤体形成、线粒体浓度和基因表达模式来促进其 IVF 胚胎发育能力。