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Water-transport and intracellular ice formation of human adipose-derived stem cells during freezing
Journal of Thermal Biology ( IF 2.7 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.jtherbio.2020.102689
Zifei Li 1 , Lingxiao Shen 2 , Yu Huang 3 , Xingxue Xiang 2 , Gang Zhao 2 , Jie Luan 1
Affiliation  

BACKGROUND Water transport and intracellular ice formation are important processes that relate to cryoinjury of cells upon freezing. To date, no study is reported on the characteristics of water transport and intracellular ice formation in porcine adipose-derived stem cells (pADSC). OBJECTIVE To study water transport and intracellular ice formation upon freezing of pADSCs at different cooling rates. MATERIALS AND METHODS The pADSCs were isolated using collagenase digestion from a subcutaneous adipose tissue of a 28-day-old Landrace pig. Freeze experiments were performed in a gas tight chamber of cryomicroscopy stage at different cooling rates between 40°C and -150°C. RESULTS Water permeability coefficient Lpg and the activation energy ELP decrease with increasing cooling rates for pADSCs. The probability of intracellular ice formation increases with increasing cooling rates, being 0.35, 0.4 and 0.5 for cooling rates at 20, 30 and 60 °C/min respectively. CONCLUSION Based on the characteristics of water transport and intracellular ice formation in pADSCs, slow freezing is perhaps more suitable for pADSC cryopreservation.

中文翻译:

人脂肪干细胞在冷冻过程中的水运输和细胞内冰形成

背景水运输和细胞内冰形成是与冷冻时细胞的低温损伤相关的重要过程。迄今为止,还没有关于猪脂肪来源干细胞(pADSC)中水分运输和细胞内冰形成特征的研究。目的研究pADSCs在不同冷却速率下冷冻后水的运输和细胞内冰的形成。材料与方法 使用胶原酶消化法从 28 日龄长白猪的皮下脂肪组织中分离出 pADSC。冷冻实验在低温显微镜阶段的气密室中以 40°C 和 -150°C 之间的不同冷却速率进行。结果 水渗透系数 Lpg 和活化能 ELP 随着 pADSCs 冷却速率的增加而降低。细胞内冰形成的概率随着冷却速率的增加而增加,冷却速率分别为 0.35、0.4 和 0.5,分别为 20、30 和 60 °C/min。结论基于pADSCs的水分运输和细胞内结冰的特点,慢速冷冻可能更适合pADSCs的冷冻保存。
更新日期:2020-10-01
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