ABSTRACT: Marinobacter comprises a widespread and ecologically significant genus of marine bacteria with vast metabolic capabilities. However, very few reports on phages infecting this genus exist. In this study, we isolated and characterized a linear dsDNA genome of Marinobacter phage AS1, infecting Marinobacter sp. strain D1S9. This phage, which is a member of the family Podoviridae, has a genome size of ~37 kb and a 57% GC content, with no significant overall similarity to any of the available viral whole genome sequences in the database. The genome, which encodes for 52 protein-coding genes with no tRNA genes, contains 21 protein-coding genes with assigned putative functions. A high degree of recombination events with other members of the order Caudovirales is evident from the mosaic pattern of inheritance of genes encoding major capsid protein, terminase large sub unit, portal protein and integrase. The presence of the integrase gene, DNA methyl transferases and the occurrence of AS1 sequences within the whole genome sequences of some members of the host taxon are suggestive of its intense association with the host.

中文翻译：

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Marinobacter噬菌体AS1的基因组分析表明，它与宿主Marinobacter sp。密切相互作用。

摘要：Marinobacter包含具有广泛代谢功能的广泛且具有生态学意义的海洋细菌属。但是，关于噬菌体感染该属的报道很少。在这项研究中，我们分离并鉴定了感染Marinobacter sp。的Marinobacter噬菌体AS1的线性dsDNA基因组。菌株D1S9。该噬菌体是Podoviridae家族的成员的基因组大小约为〜37 kb，GC含量为57％，与数据库中任何可用的病毒全基因组序列均无显着的总体相似性。该基因组编码52个没有tRNA基因的蛋白质编码基因，包含21个具有指定推定功能的蛋白质编码基因。从编码主要衣壳蛋白，末端酶大亚基，门户蛋白和整合酶的基因的遗传镶嵌模式，可以清楚地看出与假单胞菌其他成员的高度重组事件。整合酶基因，DNA甲基转移酶的存在以及宿主分类单元某些成员的全基因组序列中AS1序列的出现暗示了它与宿主的紧密联系。