ABSTRACT: Cold groundwater springs at the edges of lava fields along the volcanic active zone in Iceland are an interesting habitat, presenting an ecotone between groundwater, surface water and the terrestrial ecosystems. They are categorized as fennoscandian mineral-rich springs according to the European Nature Information System (EUNIS) classification (C2.111 European Environmental Agency) and have a high conservation value. They are also an island-like phenomena in the landscape and, together with the stable chemical and physical properties of the groundwater, make excellent study sites for testing questions regarding community assembly theory. To explore the biota of these systems, we applied environmental metabarcoding to assess ciliate diversity in this habitat. DNA was extracted and metabarcoding based on the 18S rRNA gene was conducted for (1) water samples and (2) glass beads as support for biomass development. Alpha diversity for ciliate communities in the spring sources increased with temperature, and limnocrene springs had fewer, more abundant taxa than rheocrene springs. Differences were observed between the water samples and the glass bead samples, mainly in terms of abundance. When considering only the water samples, no variation was found among spring source communities, indicating that stochastic processes such as dispersal and ecological drift might be important in shaping the community composition.

中文翻译：

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冰岛冷水泉源的纤毛虫多样性

摘要：冰岛火山活动区熔岩田边缘的冷地下水泉水是一个有趣的栖息地，是地下水，地表水和陆地生态系统之间的一个过渡带。根据欧洲自然信息系统（EUNIS）分类（C2.111欧洲环保署），它们被归类为芬诺斯堪的纳富含矿物质的泉水，并且具有很高的保护价值。它们也是景观中的岛状现象，并且与地下水的稳定化学和物理特性一起，成为测试有关社区装配理论的问题的绝佳研究场所。为了探索这些系统的生物区系，我们应用了环境元条形码来评估该生境中的纤毛虫多样性。提取DNA并基于18S rRNA基因对（1）水样品和（2）玻璃珠进行生物条形码编码，以支持生物质的发展。春季源中纤毛虫群落的α多样性随温度增加而增加，而丽角cre泉的类群少于角cre泉。观察到水样品和玻璃珠样品之间的差异，主要是在丰度方面。仅考虑水样时，在泉源社区之间未发现任何变化，这表明诸如分散和生态漂移之类的随机过程对于塑造社区组成可能很重要。比rhecrene泉水更丰富的分类单元。观察到水样品和玻璃珠样品之间的差异，主要是在丰度方面。仅考虑水样时，在泉源社区之间未发现任何变化，这表明诸如分散和生态漂移之类的随机过程对于塑造社区组成可能很重要。比rhecrene泉水更丰富的分类单元。观察到水样品和玻璃珠样品之间的差异，主要是在丰度方面。仅考虑水样时，在泉源社区之间未发现任何变化，这表明诸如分散和生态漂移之类的随机过程对于塑造社区组成可能很重要。