ABSTRACT: Detailed knowledge about basic metabolism in the early life stages of fishes helps increase our understanding of energetically important life phases. However, little is known about metabolic capacities during ontogenetic development of anemone fishes before and after settlement. This study established activities of 3 key metabolic enzymes—citrate synthase (CS), pyruvate kinase (PK) and lactate dehydrogenase (LDH)—to establish oxidative and glycolytic capacities in eggs, larvae and juveniles of false clownfish Amphiprion ocellaris before and after hatch, and during ontogenetic metamorphosis from the pelagic to sedentary mode of life (settlement stage). Oxidative capacity significantly increased after hatching and remained constant throughout development. Compared to just-hatched larvae (1-2 d old), the glycolytic and fermentative capacities on the contrary were about 2.5- and 2.1-fold higher in pre-settlement (7-8 d old) larvae, respectively, and decreased significantly in post-settlement (15 d old) juveniles. Thus, relative glycolytic enzyme activity ratios verified that settlement-stage anemone fish larvae mostly rely on glycolytic capacities for the burst-swimming capabilities needed during settlement before entering into the benthic mode of life. Intraspecific variances in enzyme activities due to ontogenetic transition should be considered when using key metabolic enzymes as biomarkers for analysing larval physiological status.

中文翻译：

---

糖酵解能力取决于小丑鱼两栖动物的发育阶段

摘要：有关鱼类早期生命阶段基本代谢的详细知识有助于增进我们对能量上重要的生命阶段的了解。但是，关于海葵鱼类在定居前后的发育过程中的代谢能力知之甚少。这项研究建立了柠檬酸合酶（CS），丙酮酸激酶（PK）和乳酸脱氢酶（LDH）的3种关键代谢酶的活性，以建立假小丑鱼两栖类双球菌卵，卵和幼虫中的氧化和糖酵解能力。孵化前后，从中上生到久坐的生活方式（安居阶段）的个体发育变态。孵化后的氧化能力显着提高，并且在整个发育过程中保持恒定。与刚孵化的幼虫（1-2天大）相比，沉降前（7-8天大）的幼虫的糖酵解能力和发酵能力分别高约2.5倍和2.1倍，而在孵化前的幼虫中则显着降低。结算后（15天大）的少年。因此，相对的糖酵解酶活性比值证明，沉降阶段海葵鱼的幼虫在进入底栖生活模式之前，主要依靠糖酵解能力来解决沉降期间所需的爆发游泳能力。