ABSTRACT: Most research into the effects of phytochemical isoflavones has focussed on endocrine disruptions, and especially on oestrogenic imbalances; however, little is known about their effects on other molecular signals such as transcriptional coregulators and choriolytic enzymatic pathways, which are also important in reproductive processes. In male and female zebrafish Danio rerio, the soya isoflavones genistein and daidzein (provided at 10 mg l^-1 for 15 d) modulated the basal expression levels of oestrogen receptor transcripts (ERβ) in variable and differential ways. Exposure to genistein resulted in decreased levels of ERβ in the zebrafish ovary; conversely, this isoflavone increased the basal expression levels of the hatching enzyme (HE1) in both gonads. On the other hand, daidzein increased the basal expression levels of the bromodomain testis-specific gene (BRDT) in the male gonad, but not in the ovary. Both isoflavones also differentially modulated (up-down regulations) the basal expression patterns of the 3 molecular signals studied in other regions of the body (e.g. head, digestive system, skeletal musculature). Despite all these transcriptional imbalances, neither of the phytoestrogens modified gonadal histomorphology or the baseline histochemical pattern of proteins, carbohydrates and glycoconjugates distributed in either the vitelline structures or in the developing and maturing germ cells of Danio rerio.

中文翻译：

---

大豆异黄酮，金雀异黄素和黄豆苷元在斑马鱼丹尼奥里奥的卵巢和睾丸中诱导差异转录调节

摘要：大多数关于植物化学异黄酮作用的研究都集中在内分泌干扰，特别是雌激素失衡上。然而，关于它们对其他分子信号（如转录共调节因子和绒毛膜溶酶途径）的影响知之甚少，这在生殖过程中也很重要。在雄性和雌性斑马鱼Danio rerio中，大豆异黄酮染料木黄酮和大豆苷元（以10 mg l ^-1提供）15 d）以可变和差异方式调节雌激素受体转录本（ERβ）的基础表达水平。暴露于染料木黄酮会导致斑马鱼卵巢中的ERβ水平降低；相反，该异黄酮增加了两个性腺中孵化酶（HE1）的基础表达水平。另一方面，黄豆苷元在雄性腺中增加了溴结构域睾丸特异性基因（BRDT）的基础表达水平，但在卵巢中却没有。两种异黄酮还对人体其他部位（例如头部，消化系统，骨骼肌组织）研究的3种分子信号的基础表达模式进行差异调节（上下调节）。尽管存在所有这些转录失衡，但植物雌激素均不能改变性腺的组织形态学或蛋白质的基线组织化学模式，Danio rerio。