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Basil seeds as a source of antioxidants affected by fortification with selenium
Folia Horticulturae ( IF 2 ) Pub Date : 2020-04-18 , DOI: 10.2478/fhort-2020-0002
Ivana Mezeyová 1 , Alžbeta Hegedűsová 1 , Ondrej Hegedűs 2 , Andrea Vargová 3 , Mária Timoracká 4 , Miroslav Šlosár 1 , Alena Andrejiová 1 , Tünde Juríková 5 , Ján Mezey 6
Affiliation  

The work aimed to determine the potential of selenium incorporation into seeds of selected species of Ocimum spp. after fortification with a foliar solution of sodium selenate at a concentration of 50 g Se · ha−1. In a 2-year trial, the selenium content was determined by electrothermal atomic absorption method with Zeeman background correction. Modified spectrophotometric method (2,2-diphenyl-1-picrylhydrazyl [DPPH] assays) was used to rate the potential of oxidation– reduction components of basil seeds (AA). The total polyphenol content (TPC) was determined spectrophotometrically using the Folin–Ciocalteu reagent and gallic acid (GA) as the standard solution. The results of experiments showed that the selenium biofortification significantly (p < 0.05) increased the content of selenium in basil seeds (17-fold increase in comparison with controlled variant in case of Tulsi, 12-fold in ‘Cinamonette’ and 12-fold in ‘Dark Green’ when compared with control). The basil seeds represented a valuable source of polyphenols (1414.61–1681.75 μg GA · g−1 dried weight [d.w.]) with multiple times higher antioxidant activity (23.50–28.97 mmol Trolox · kg−1) in comparison with common tested horticultural crops (e.g. peas, tomato and pumpkin). Significant influence of fortification was not found in AA and TPC values. Fortification was not significantly reflected in AA and TPC values. In addition to its very strong reproductive function, healing and religious purposes, the basil seed is used as a functional food due to its high content of bioactive compounds.

中文翻译:

罗勒种子作为抗氧化剂的来源,受硒强化的影响

这项工作旨在确定硒掺入选定的罗勒属物种的种子中的潜力。用浓度为50 g Se·ha -1的硒酸钠叶面溶液强化后。在一项为期2年的试验中,采用Zeeman背景校正通过电热原子吸收法测定了硒含量。改良的分光光度法(2,2-二苯基-1-吡啶并肼基[DPPH]测定)用于评估罗勒种子(AA)氧化还原成分的潜力。使用Folin–Ciocalteu试剂和没食子酸(GA)作为标准溶液,通过分光光度法测定总多酚含量(TPC)。实验结果表明,硒的生物强化作用显着(p<0.05)提高了罗勒种子中硒的含量(与对照相比,相比于Tulsi对照变种增加了17倍,“ Cinamonette”为12倍,“深绿色”为12倍)。罗勒种子是多酚(1414.61–1681.75μgGA·g -1干重[dw])的重要来源,具有较高的抗氧化活性(23.50–28.97 mmol Trolox·kg -1)与经过测试的常见园艺作物(例如豌豆,番茄和南瓜)相比。在AA和TPC值中未发现防御工事的重大影响。在AA和TPC值中未显着强化。罗勒种子除具有非常强的生殖功能,治愈和宗教用途外,还由于其高含量的生物活性化合物而被用作功能性食品。
更新日期:2020-04-18
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