Cistus crispus (Cistaceae) is a species adapted to arid and semi-arid conditions, and it has ornamental and medicinal uses. In Italy, native populations of C. crispus are threatened by the collection due to the low number of individuals in the populations, anthropogenic pressure, and the changing environmental condition that enhance other more aggressive and hybridisable Cistus species. Here, we set up the variables for its micropropagation protocols to achieve a high number of plants per unit time. Various steps of the micropropagation protocol were modulated, including various sodium hypochlorite concentrations (CHCs) and time of sterilisation. The efficiency of the protocol maximised at 25 min sterilisation with 2.5% CHC and decreasing explant vitality at increasing time and CHC. Both shoot proliferation and root emissions were maximised at 1.78 μM N-6-benzyladenine (BA) in the growing medium, with up to 5.4 explants per cycle, with 5.8 roots per explant, and 84 healthy explants. Kinetin stimulated further the axillary root proliferation more than dimethylallylamino purine or BA. Lastly, the application of indole acetic acid increased root emissions during the acclimation stage more than the application of indole butyric acid, and this occurred irrespective of their concentrations, up to 2.0 μg · g⁻¹. These results can foster the use of C. crispus as an ornamental species, for xeriscaping or for the extraction of its secondary compounds, which have various industrial uses. These results can also have an indirect implication for the conservation of the species by reducing the collection for ornamental purposes from its natural population.

中文翻译：

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Cistus crispus L.（Cistaceae）微繁殖的有效方案

Cistus crispus（Cistaceae）是一种适应干旱和半干旱条件的物种，具有观赏和药用用途。在意大利，由于种群中的个体数量少，人为压力以及不断变化的环境条件（增强了其他更具侵略性和杂交性的水仙），该菌种受到当地种群的威胁。种类。在这里，我们为其微繁协议设置了变量，以在单位时间内获得大量植物。调节了微繁殖方案的各个步骤，包括各种次氯酸钠浓度（CHC）和灭菌时间。该方案的效率在25％灭菌（2.5％CHC）时最大化，并在增加时间和CH​​C时降低外植体活力。两个芽增殖和根排放量在1.78μM最大化Ñ生长培养基中的-6-苄基腺嘌呤（BA），每个周期最多有5.4个外植体，每个外植体有5.8个根，而健康外植体有84个。激动素比二甲基烯丙基氨基嘌呤或BA进一步刺激腋窝根部增殖。最后，在适应阶段，吲哚乙酸的施用比吲哚丁酸的施用增加了更多的根排放，而不论其浓度如何，这种排放的发生均高达2.0μg·g ^-1。这些结果可以促进C. crispus的使用作为观赏植物，用于干馏或提取其次要化合物，具有多种工业用途。这些结果也可能通过减少自然种群中用于观赏目的的收集而对物种的保护产生间接影响。