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Aligned collagen scaffold combination with human spinal cord-derived neural stem cells to improve spinal cord injury repair.
Biomaterials Science ( IF 6.6 ) Pub Date : 2020-08-20 , DOI: 10.1039/d0bm00431f
Yunlong Zou 1 , Dezun Ma , He Shen , Yannan Zhao , Bai Xu , Yongheng Fan , Zheng Sun , Bing Chen , Weiwei Xue , Ya Shi , Zhifeng Xiao , Rui Gu , Jianwu Dai
Affiliation  

Neural stem/progenitor cell (NSPC)-based spinal cord injury (SCI) therapy is expected to bridge the lesion site by transplanting exogenous NSPCs for replacement of lost cells. The transplanted NSPCs produce a microenvironment conducive to neuronal regeneration, and ultimately, functional recovery. Although both human fetal brain- and spinal cord- derived NSPCs (hbNSPCs and hscNSPCs, respectively) have been used for SCI repair, it remains unclear whether hscNSPCs are a more appropriate stem cell source for transplantation than hbNSPCs. Therefore, in this study, we transplanted hbNSPCs or hscNSPCs into rats with complete transection SCI to monitor their differences in SCI treatment. An aligned collagen sponge scaffold (ACSS) was used here for cell retention. Aligned biomaterial scaffolds provide a support platform and favorable morphology for cell growth and differentiation, and guide axial axonal extension. The ACSS fabricated by our group has been previously reported to improve spinal cord repair by promoting neuronal regeneration and remyelination. Compared with the hbNSPC–ACSS, the hscNSPC–ACSS effectively promoted long-term cell survival and neuronal differentiation and improved the SCI microenvironment by reducing inflammation and glial scar formation. Furthermore, the transplanted hscNSPC–ACSS improved recovery of locomotor functions. Therefore, hscNSPCs appear to be a superior cell source to hbNSPCs for SCI cell therapy with greater potential clinical applications.

中文翻译:

对齐的胶原蛋白支架与人类脊髓源性神经干细胞的组合,以改善脊髓损伤的修复。

基于神经干/祖细胞(NSPC)的脊髓损伤(SCI)治疗有望通过移植外源NSPC替代丢失的细胞来桥接病变部位。移植的NSPC产生有利于神经元再生并最终恢复功能的微环境。尽管人类胎儿脑和脊髓来源的NSPC(分别为hbNSPC和hscNSPC)都已用于SCI修复,但仍不清楚hscNSPC是否比hbNSPC更适合用于移植干细胞。因此,在这项研究中,我们将hbNSPC或hscNSPC移植到具有完全横断SCI的大鼠中,以监测它们在SCI治疗中的差异。对准的胶原海绵支架(ACSS)在这里用于细胞保留。对齐的生物材料支架为细胞生长和分化提供了支撑平台和良好的形态,并指导了轴突的轴向延伸。先前已经报道了由我们小组制造的ACSS通过促进神经元再生和髓鞘再生来改善脊髓修复。与hbNSPC–ACSS相比,hscNSPC–ACSS有效地促进了长期细胞存活和神经元分化,并通过减少炎症和神经胶质瘢痕形成改善了SCI微环境。此外,移植的hscNSPC-ACSS改善了运动功能的恢复。因此,hscNSPC似乎是用于SCI细胞治疗的hbNSPC的优越细胞来源,具有更大的潜在临床应用。先前已经报道了由我们小组制造的ACSS通过促进神经元再生和髓鞘再生来改善脊髓修复。与hbNSPC–ACSS相比,hscNSPC–ACSS有效地促进了长期细胞存活和神经元分化,并通过减少炎症和神经胶质瘢痕形成改善了SCI微环境。此外,移植的hscNSPC-ACSS改善了运动功能的恢复。因此,hscNSPC似乎是用于SCI细胞治疗的hbNSPC的优越细胞来源,具有更大的潜在临床应用。先前已经报道了由我们小组制造的ACSS通过促进神经元再生和髓鞘再生来改善脊髓修复。与hbNSPC–ACSS相比,hscNSPC–ACSS有效地促进了长期细胞存活和神经元分化,并通过减少炎症和神经胶质瘢痕形成改善了SCI微环境。此外,移植的hscNSPC-ACSS改善了运动功能的恢复。因此,hscNSPC似乎是用于SCI细胞治疗的hbNSPC的优越细胞来源,具有更大的潜在临床应用。hscNSPC–ACSS通过减少炎症和神经胶质瘢痕形成,有效促进了长期细胞存活和神经元分化,并改善了SCI微环境。此外,移植的hscNSPC-ACSS改善了运动功能的恢复。因此,hscNSPC似乎是用于SCI细胞治疗的hbNSPC的优越细胞来源,具有更大的潜在临床应用。hscNSPC–ACSS通过减少炎症和神经胶质瘢痕形成,有效促进了长期细胞存活和神经元分化,并改善了SCI微环境。此外,移植的hscNSPC-ACSS改善了运动功能的恢复。因此,hscNSPC似乎是用于SCI细胞治疗的hbNSPC的优越细胞来源,具有更大的潜在临床应用。
更新日期:2020-09-15
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