DEAD-box proteins (DBPs) are RNA remodeling factors associated with RNA helicase activity that are found in nearly all organisms. Despite extensive studies on the mechanisms used by DBPs to regulate RNA function, very little is known about how DBPs themselves are regulated. In this work, we have analyzed the expression and regulation of DeaD/CsdA, the largest of the DBPs in Escherichia coli (E. coli). We show that deaD transcription initiates 838 nts upstream of the start of the coding region. We have also found that DeaD is autoregulated through a negative feedback mechanism that operates both at the level of mRNA stability and Rho-dependent transcription termination, and this regulation is dependent upon its mRNA 5' untranslated region (5' UTR). These findings suggest that DeaD might be regulating the conformation of its own mRNA through its RNA helicase activity to facilitate ribonuclease and Rho access to its 5' UTR.

中文翻译：

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通过 mRNA 稳定性和 Rho 依赖性转录终止对大肠杆菌 DeaD RNA 解旋酶进行双水平自动调节

DEAD-box 蛋白 (DBP) 是与 RNA 解旋酶活性相关的 RNA 重构因子，几乎在所有生物体中都有发现。尽管对 DBPs 用于调节 RNA 功能的机制进行了广泛的研究，但对 DBPs 本身如何调节知之甚少。在这项工作中，我们分析了 DeaD/CsdA（大肠杆菌中最大的 DBP）的表达和调控。我们表明，deaD 转录在编码区起点上游 838 nts 处启动。我们还发现，DeaD 是通过负反馈机制自动调节的，该机制在 mRNA 稳定性和 Rho 依赖性转录终止水平上起作用，并且这种调节依赖于其 mRNA 5' 非翻译区 (5' UTR)。