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Intravital longitudinal imaging of hepatic lipid droplet accumulation in a murine model for nonalcoholic fatty liver disease
Biomedical Optics Express ( IF 3.4 ) Pub Date : 2020-08-19 , DOI: 10.1364/boe.395890 Jieun Moon , Eunji Kong , Jingu Lee , Jinjoo Jung , Eunha Kim , Seung Bum Park , Pilhan Kim
Biomedical Optics Express ( IF 3.4 ) Pub Date : 2020-08-19 , DOI: 10.1364/boe.395890 Jieun Moon , Eunji Kong , Jingu Lee , Jinjoo Jung , Eunha Kim , Seung Bum Park , Pilhan Kim
Nonalcoholic fatty liver disease (NAFLD) is a rapidly increasing chronic liver disorder worldwide accompanied by hepatic steatosis, inflammation, fibrosis, and severe liver failure. Unfortunately, an effective treatment strategy for NAFLD has not yet been established, which has been hampered by the limited understanding of the pathophysiological drivers for NAFLD. To examine the unknown cellular and molecular mechanisms in the pathogenesis of NAFLD, there is an increasing need for the direct in vivo observation of hepatic microenvironments over extended periods of time. In this work, using a custom-built intravital imaging system and a novel fluorescent lipid droplet labeling dye, Seoul-Fluor 44 (SF44), we established an intravital imaging method to visualize individual lipid droplets and microvasculature simultaneously in the liver of live mice in vivo. In addition, in the nonalcoholic steatosis and steatohepatitis mouse model induced by a methionine and choline-deficient diet, we longitudinally visualized and quantitatively analyzed the development of lipid droplets in hepatocytes and sinusoid at a subcellular resolution during the progression of NAFLD up to 21 days in vivo.
中文翻译:
非酒精性脂肪性肝病小鼠模型中肝脂质滴积累的玻璃体内纵向成像
非酒精性脂肪性肝病(NAFLD)是全世界范围内迅速增加的慢性肝病,伴有肝脂肪变性,炎症,纤维化和严重的肝衰竭。不幸的是,尚未建立针对NAFLD的有效治疗策略,这由于对NAFLD的病理生理驱动因素的了解有限而受到阻碍。为了检查NAFLD发病机理中未知的细胞和分子机制,对直接体内的需求日益增长长时间观察肝微环境。在这项工作中,我们使用定制的活体内成像系统和新型荧光脂质滴标记染料Seoul-Fluor 44(SF44),建立了一种活体成像方法来同时可视化活小鼠肝脏中的单个脂质滴和微脉管系统。体内。此外,在由蛋氨酸和胆碱缺乏饮食引起的非酒精性脂肪变性和脂肪性肝炎小鼠模型中,我们以可视化的方式纵向观察并定量分析了NAFLD长达21天的过程中亚细胞分辨率下肝细胞和正弦曲线中脂质滴的形成。体内。
更新日期:2020-09-01
中文翻译:
非酒精性脂肪性肝病小鼠模型中肝脂质滴积累的玻璃体内纵向成像
非酒精性脂肪性肝病(NAFLD)是全世界范围内迅速增加的慢性肝病,伴有肝脂肪变性,炎症,纤维化和严重的肝衰竭。不幸的是,尚未建立针对NAFLD的有效治疗策略,这由于对NAFLD的病理生理驱动因素的了解有限而受到阻碍。为了检查NAFLD发病机理中未知的细胞和分子机制,对直接体内的需求日益增长长时间观察肝微环境。在这项工作中,我们使用定制的活体内成像系统和新型荧光脂质滴标记染料Seoul-Fluor 44(SF44),建立了一种活体成像方法来同时可视化活小鼠肝脏中的单个脂质滴和微脉管系统。体内。此外,在由蛋氨酸和胆碱缺乏饮食引起的非酒精性脂肪变性和脂肪性肝炎小鼠模型中,我们以可视化的方式纵向观察并定量分析了NAFLD长达21天的过程中亚细胞分辨率下肝细胞和正弦曲线中脂质滴的形成。体内。