Increasing evidence suggest that bacteria form diverse communities in various eukaryotic hosts, including fungi. However, little is known about their succession and the functional potential at different host development stages. Here we examined the effect of fruiting body parts and developmental stages on the structure and potential function of fungus-associated bacterial communities. Using high-throughput sequencing, we characterized bacterial communities and their associated potential functions in fruiting bodies from ten genera belonging to four major mushroom-forming orders and three different developmental stages of a model host species Cantharellus cibarius. Our results demonstrate that bacterial community structure differs between internal and external parts of the fruiting body but not between inner tissues. The structure of the bacterial communities showed significant variation across fruiting body developmental stages. We provide evidence that certain functional groups, such as those related to nitrogen fixation, persist in fruiting bodies during the maturation, but are replaced by putative parasites/pathogens afterwards. These data suggest that bacterial communities inhabiting fungal fruiting bodies may play important roles in their growth and development.

中文翻译：

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真菌子实体发育阶段的细菌群落动态。

越来越多的证据表明，细菌在包括真菌在内的各种真核宿主中形成了不同的群落。然而，关于它们在不同宿主发育阶段的继承和功能潜力知之甚少。在这里，我们检查了子实体部分和发育阶段对真菌相关细菌群落的结构和潜在功能的影响。使用高通量测序，我们表征了十个属的子实体中的细菌群落及其相关的潜在功能，这些属来自四个主要蘑菇形成顺序和三个不同发育阶段的模型宿主物种Cantharellus cibarius。我们的结果表明，子实体的内部和外部之间的细菌群落结构不同，但内部组织之间没有差异。细菌群落的结构在子实体发育阶段显示出显着变化。我们提供的证据表明，某些功能基团（例如与固氮相关的功能基团）在成熟过程中会保留在子实体中，但之后被假定的寄生虫/病原体所取代。这些数据表明，居住在真菌子实体上的细菌群落可能在其生长发育中发挥重要作用。