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Over-expression of arginine vasopressin in magnocellular neurosecretory cells of hypothalamus and its potential relationship with development of diabetic nephropathy.
Archives of Medical Science ( IF 3.8 ) Pub Date : 2020-01-19 , DOI: 10.5114/aoms.2020.92402 Xianhua Li 1 , Zhaoli Gao 2 , Ling Xu 2 , Baoying Li 2 , Haiqing Gao 2
Archives of Medical Science ( IF 3.8 ) Pub Date : 2020-01-19 , DOI: 10.5114/aoms.2020.92402 Xianhua Li 1 , Zhaoli Gao 2 , Ling Xu 2 , Baoying Li 2 , Haiqing Gao 2
Affiliation
Introduction
We aimed to assess our hypothesis that the expression changes of arginine vasopressin (AVP) in the magnocellular neurosecretory cells (MNCs) of hypothalamus and V2 receptor for AVP (RVP) in kidney may contribute to the pathogenesis of diabetic nephropathy (DN).
Material and methods
Twenty-five male Wistar rats were randomly assigned to the control group and the diabetes mellitus (DM) group. Periodic acid-Schiff (PAS) staining and electron microscopy were used for morphological studies. Immunohistochemical staining for glial fibrillary acidic protein (GFAP) is standard for visualization of reactive astrocytes in the hypothalamus. Hypothalamus was used for immunofluorescence of AVP. Kidney was used for immunohistochemical staining of RVP. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was used for quantitative determinations of AVP mRNA in hypothalamus and RVP mRNA in kidney. Western blot was used to measure the protein expression of AVP in hypothalamus and RVP in kidney.
Results
Morphological studies showed abnormalities in kidney and hypothalamus in the DM group. The number of neurons and the gray value of astrocytes in hypothalamus in the DM group were markedly decreased. The expression level of AVP in hypothalamus and the expression level of RVP in kidney of DM rats were significantly increased. The positive correlations between the proteinuria and expression (mRNA and protein) of AVP, proteinuria and expression (mRNA and protein) of RVP, and the expression of AVP and RVP levels were found.
Conclusions
AVP was upregulated in the MNCs of hypothalamus and RVP was upregulated in kidney in streptozotocin-induced DM rats, indicating their potential roles in the development of DN.
中文翻译：
下丘脑大细胞神经分泌细胞中精氨酸加压素的过表达及其与糖尿病肾病发生发展的潜在关系。
引言
我们旨在评估我们的假设，即精氨酸加压素 (AVP) 在下丘脑的大细胞神经分泌细胞 (MNC) 和肾脏中 AVP 的 V2 受体 (RVP) 的表达变化可能有助于糖尿病肾病 (DN) 的发病机制。
材料与方法
将 25 只雄性 Wistar 大鼠随机分配到对照组和糖尿病 (DM) 组。高碘酸-希夫 (PAS) 染色和电子显微镜用于形态学研究。胶质纤维酸性蛋白 (GFAP) 的免疫组织化学染色是下丘脑反应性星形胶质细胞可视化的标准方法。下丘脑用于 AVP 的免疫荧光。肾脏用于RVP的免疫组织化学染色。定量实时逆转录聚合酶链反应 (qRT-PCR) 用于定量测定下丘脑中的 AVP mRNA 和肾脏中的 RVP mRNA。Western blot检测下丘脑AVP和肾脏RVP蛋白表达。
结果
形态学研究显示 DM 组肾脏和下丘脑异常。DM组下丘脑神经元数量和星形胶质细胞灰度值明显降低。DM大鼠下丘脑AVP表达水平和肾脏RVP表达水平明显升高。发现蛋白尿与AVP表达（mRNA和蛋白）、蛋白尿与RVP表达（mRNA和蛋白）、AVP和RVP水平表达呈正相关。
结论
链脲佐菌素诱导的 DM 大鼠下丘脑 MNCs 中 AVP 表达上调，肾脏 RVP 表达上调，提示其在 DN 发生发展中的潜在作用。
更新日期：2020-01-19
We aimed to assess our hypothesis that the expression changes of arginine vasopressin (AVP) in the magnocellular neurosecretory cells (MNCs) of hypothalamus and V2 receptor for AVP (RVP) in kidney may contribute to the pathogenesis of diabetic nephropathy (DN).
Material and methods
Twenty-five male Wistar rats were randomly assigned to the control group and the diabetes mellitus (DM) group. Periodic acid-Schiff (PAS) staining and electron microscopy were used for morphological studies. Immunohistochemical staining for glial fibrillary acidic protein (GFAP) is standard for visualization of reactive astrocytes in the hypothalamus. Hypothalamus was used for immunofluorescence of AVP. Kidney was used for immunohistochemical staining of RVP. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was used for quantitative determinations of AVP mRNA in hypothalamus and RVP mRNA in kidney. Western blot was used to measure the protein expression of AVP in hypothalamus and RVP in kidney.
Results
Morphological studies showed abnormalities in kidney and hypothalamus in the DM group. The number of neurons and the gray value of astrocytes in hypothalamus in the DM group were markedly decreased. The expression level of AVP in hypothalamus and the expression level of RVP in kidney of DM rats were significantly increased. The positive correlations between the proteinuria and expression (mRNA and protein) of AVP, proteinuria and expression (mRNA and protein) of RVP, and the expression of AVP and RVP levels were found.
Conclusions
AVP was upregulated in the MNCs of hypothalamus and RVP was upregulated in kidney in streptozotocin-induced DM rats, indicating their potential roles in the development of DN.
中文翻译：
下丘脑大细胞神经分泌细胞中精氨酸加压素的过表达及其与糖尿病肾病发生发展的潜在关系。
引言
我们旨在评估我们的假设，即精氨酸加压素 (AVP) 在下丘脑的大细胞神经分泌细胞 (MNC) 和肾脏中 AVP 的 V2 受体 (RVP) 的表达变化可能有助于糖尿病肾病 (DN) 的发病机制。
材料与方法
将 25 只雄性 Wistar 大鼠随机分配到对照组和糖尿病 (DM) 组。高碘酸-希夫 (PAS) 染色和电子显微镜用于形态学研究。胶质纤维酸性蛋白 (GFAP) 的免疫组织化学染色是下丘脑反应性星形胶质细胞可视化的标准方法。下丘脑用于 AVP 的免疫荧光。肾脏用于RVP的免疫组织化学染色。定量实时逆转录聚合酶链反应 (qRT-PCR) 用于定量测定下丘脑中的 AVP mRNA 和肾脏中的 RVP mRNA。Western blot检测下丘脑AVP和肾脏RVP蛋白表达。
结果
形态学研究显示 DM 组肾脏和下丘脑异常。DM组下丘脑神经元数量和星形胶质细胞灰度值明显降低。DM大鼠下丘脑AVP表达水平和肾脏RVP表达水平明显升高。发现蛋白尿与AVP表达（mRNA和蛋白）、蛋白尿与RVP表达（mRNA和蛋白）、AVP和RVP水平表达呈正相关。
结论
链脲佐菌素诱导的 DM 大鼠下丘脑 MNCs 中 AVP 表达上调，肾脏 RVP 表达上调，提示其在 DN 发生发展中的潜在作用。
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