ABSTRACT

Objective

We aimed to investigate the inhibitory effect of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer on the adherence of Streptococcus pneumoniae (Spn) and nontypeable Haemophilus influenzae (NTHi) in vitro and in vivo.

Materials and Methods

Phosphorylcholine (PC) expression of 21 strains each of Spn and NTHi was evaluated using fluorescence-activated cell sorting; the adherence of bacteria to Detroit 562 cells and to the nasal mucosa of BALB/c mice was determined. MPC polymer-mediated inhibitory effects were compared with PC-keyhole limpet hemocyanin (PC-KLH)-mediated inhibitory effects.

Results

In vitro experiments showed that pretreatment with MPC polymer markedly inhibited the adherence of Spn and NTHi in a concentration dose–dependent manner independently of PC expression. No correlation was observed between PC expression and MPC polymer-mediated inhibitory effects. Contrarily, there was a significant negative correlation between PC-KLH-mediated inhibitory effects and PC expression in Spn and NTHi. The same results were obtained via in vivo experiments. The MPC polymer did not affect the histology of the nasal mucosa.

Conclusions

MPC polymer might be effective to reduce the occurrence of upper respiratory tract infection caused by Spn and NTHi and could be applied for the development of local treatments, such as topical gargles and nebulizer medications.

中文翻译：

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2-甲基丙烯酰氧基乙基磷酸胆碱聚合物对引起上呼吸道感染的细菌粘附的抑制作用

摘要

客观的

我们旨在研究 2-甲基丙烯酰氧基乙基磷酸胆碱 (MPC) 聚合物在体外和体内对肺炎链球菌(Spn) 和不可分型流感嗜血杆菌(NTHi)粘附的抑制作用。

材料和方法

使用荧光激活细胞分选法评估 Spn 和 NTHi 各 21 株的磷酸胆碱 (PC) 表达；测定了细菌对底特律 562 细胞和 BALB/c 小鼠鼻粘膜的粘附。将 MPC 聚合物介导的抑制作用与 PC 锁孔血蓝蛋白 (PC-KLH) 介导的抑制作用进行了比较。

结果

体外实验表明，用 MPC 聚合物预处理以浓度剂量依赖性方式显着抑制 Spn 和 NTHi 的粘附，与 PC 表达无关。在 PC 表达和 MPC 聚合物介导的抑制作用之间没有观察到相关性。相反，PC-KLH 介导的抑制作用与 Spn 和 NTHi 中的 PC 表达之间存在显着的负相关。通过体内实验获得了相同的结果。MPC聚合物不影响鼻粘膜的组织学。

结论

MPC 聚合物可能有效减少由 Spn 和 NTHi 引起的上呼吸道感染的发生，并可用于开发局部治疗，如局部漱口剂和雾化药物。