ABSTRACT Two bioactive peptide fractions (SWP-I and SWP-II) of swim bladder peptides from Atlantic cod (Gadus morhua) were obtained through enzymatic hydrolysis and isolated by gel filtration chromatography. The properties of two fractions were characterized by FT-IR, UV scanning spectroscopy, amino acid composition analysis and molecular weight determination. The results showed that the content of aromatic amino acids in SWP-II was higher than that in SWP-I, and the molecular of SWP-I and SWP-II were 4976 Da and 1960 Da, respectively. The radical scavenging activities showed that SWP-I and SWP-II were effective scavengers of DPPH•, HO•, and O2-•, and demonstrated high Fe2+-chelating activity. The scavenging capacities of SWP-II were stronger than those of SWP-I at the same concentration. Cytoprotective experiment suggested that SWP-I and SWP-II possessed ROS-scavenging activity. Pretreatment with these two peptides increased viability rates, suppressed SA-β-gal activities and inhibited apoptosis rates in hydrogen peroxide (H2O2)-induced premature senescent 2BS cells. In conclusion, the enzymatic peptides from swim bladders of Atlantic cod (Gadus morhua) possessed antioxidant activities and could effectively protect premature cell senescence induced by H2O2.

中文翻译：

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大西洋鳕鱼鳔肽的体外抗氧化和抗衰老特性

摘要 大西洋鳕鱼（Gadus morhua）鱼鳔肽的两种生物活性肽级分（SWP-I 和 SWP-II）通过酶水解获得，并通过凝胶过滤色谱分离。通过FT-IR、UV扫描光谱、氨基酸组成分析和分子量测定表征了两个级分的性质。结果表明，SWP-II中芳香族氨基酸的含量高于SWP-I，SWP-I和SWP-II的分子分别为4976 Da和1960 Da。自由基清除活性表明SWP-I和SWP-II是DPPH•、HO•和O2-•的有效清除剂，并表现出较高的Fe2+螯合活性。在相同浓度下，SWP-II的清除能力强于SWP-I。细胞保护实验表明，SWP-I 和 SWP-II 具有清除 ROS 的活性。用这两种肽预处理提高了存活率，抑制了 SA-β-gal 活性并抑制了过氧化氢 (H2O2) 诱导的过早衰老 2BS 细胞的凋亡率。综上所述，大西洋鳕鱼鳔酶肽具有抗氧化活性，能有效保护过氧化氢诱导的细胞过早衰老。