ABSTRACT

Association between selection pressure caused by the use of azole fungicides in sawmills and the development of fungal resistance has been described. The aim of this study was to implement an algorithm to assess the presence of Aspergillus section Fumigati resistant strains in sawmills.

Eighty-six full-shift inhalable dust samples were collected from eleven industrial sawmills in Norway. Different culture media were used and molecular identification to species level in Aspergillus section Fumigati was done by calmodulin sequencing and TR₃₄/L98H and TR₄₆/Y121F/T289A mutations were screened by real-time PCR assay and confirmed by cyp51A sequencing. Six Fumigati isolates were identified as A. fumigatus sensu stricto and two of these grew on azole-supplemented media and were further analyzed by real-time PCR. One was confirmed to be a TR₃₄/L98H mutant.

The obtained results reinforce the need to assess the presence of A. fumigatus sensu stricto resistant isolates at other workplaces with fungicide pressure.

摘要

已经描述了锯木厂使用唑类杀菌剂引起的选择压力与真菌抗性发展之间的关联。本研究的目的是实施一种算法来评估锯木厂中是否存在曲霉菌部分Fumigati抗性菌株。

从挪威的 11 个工业锯木厂收集了 86 个全班可吸入粉尘样品。使用不同的培养基，通过钙调蛋白测序对烟曲霉切片中的物种水平进行分子鉴定，通过实时PCR测定筛选TR 34 / L98H和TR 46 /Y121F/T289A突变，并_通过cyp _51A测序确认。六个Fumigatus分离株被鉴定为A. fumigatus sensu stricto，其中两个在添加唑的培养基上生长，并通过实时 PCR 进一步分析。一个被证实是TR ₃₄ /L98H 突变体。

获得的结果加强了评估A. fumigatus sensu stricto 抗性分离株在其他具有杀菌剂压力的工作场所的存在的必要性。