Multi‐enzyme cascade reactions are indispensable in biotechnology and many industrial (bio)chemical processes. However, most natural enzymes have poor stability and reusability, and tend to inactivate in toxic media or high temperature, which significantly limit their broader applications. Metal‐organic frameworks (MOFs) are promising candidates for enzymes immobilization to produce nanocomposite structures that not only could shield the enzymes from harsh environments, but also facilitate selective diffusion of substrates and intermediates to the reactive site via their tailorable and ordered pore network. Multi‐enzyme cascade reactions in MOFs have recently attracted considerable attention. This Personal Account discusses the different strategies for multi‐enzyme‐MOF interfaces and their cutting‐edge applications from biosensing and catalytic nanomedicine to artificial/hybrid cells. At last, we provide a critical evaluation and future prospects to outline future research directions.

中文翻译：

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金属有机构架中的多酶级联反应

在生物技术和许多工业（生物）化学过程中，多酶级联反应是必不可少的。但是，大多数天然酶的稳定性和重复使用性较差，并且在有毒介质或高温下易于失活，这极大地限制了其广泛的应用范围。金属有机骨架（MOF）是有望将酶固定化以产生纳米复合结构的候选人，该结构不仅可以使酶免受恶劣环境的影响，而且还可以通过其可定制和有序的孔网络将底物和中间体选择性扩散到反应位点。MOF中的多酶级联反应最近引起了相当大的关注。此个人帐户讨论了多酶MOF接口的不同策略及其在生物传感和催化纳米医学到人工/混合细胞中的前沿应用。最后，我们提供了重要的评估和未来前景，以概述未来的研究方向。