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Recombinant l‐asparaginase production using Pichia pastoris (MUTs strain): establishment of conditions for growth and induction phases
Journal of Chemical Technology and Biotechnology ( IF 3.4 ) Pub Date : 2020-07-24 , DOI: 10.1002/jctb.6540
Omar Pillaca‐Pullo 1, 2 , David Rodrigues 3 , Ignacio Sánchez‐Moguel 1 , André Lopes 4 , Marcela Pimenta 1 , Tajindar Basi 5 , Valker Feitosa 1 , Amparo Zavaleta 6 , Gisele Monteiro 1 , Adalberto Pessoa Jr 1 , Michele Vitolo 1
Affiliation  

l‐asparaginase (ASNase), a biopharmaceutical enzyme used in the treatment of childhood lymphoid malignancies, is commercially produced from Escherichia coli and Erwinia chrysanthemi. However, it causes severe adverse effects due to allergenic prokaryotic epitopes on the protein surface. ASNase II from Saccharomyces cerevisiae can be a promising alternative source of this enzyme. In this study, conditions to produce ASNase from S. cerevisiae expressed in Pichia pastoris have been investigated in shake flasks and 3 L‐bioreactor. We evaluated if medium composition, concentration of carbon source (i.e. glycerol), growth time, concentration of inducer (i.e. methanol), temperature and initial pH influenced both biomass and ASNase expression.

中文翻译:

使用巴斯德毕赤酵母(MUTs菌株)重组生产l-天冬酰胺酶:建立生长和诱导阶段的条件

l-天冬酰胺酶(ASNase)是一种用于治疗儿童淋巴恶性肿瘤的生物制药酶,由大肠杆菌菊花欧文氏菌Erwinia chrysanthemi)商业生产但是,由于蛋白表面上的致敏原核抗原决定基,它会导致严重的不良影响。来自酿酒酵母的ASNase II可能是该酶的有希望的替代来源。在这项研究中,已经在摇瓶和3 L‐中研究了从毕赤酵母中表达的酿酒酵母产生ASNase的条件生物反应器。我们评估了培养基组成,碳源(即甘油)的浓度,生长时间,诱导剂(即甲醇)的浓度,温度和初始pH是否影响生物量和ASNase表达。
更新日期:2020-07-24
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