Bacteriophages are bionanoparticles with several applications in different biotechnology‐based products. Among them, vaccines have the potential to treat antibiotic‐resistant bacteria and parasitic infections. Traditional methods for their recovery and purification rely on precipitation with polyethylene glycol (PEG) and NaCl. However, the applicability of such an approach is limited, due to large‐scale technical constrains. Recently, our research group developed a bacteriophage M13 recovery and purification strategy using Aqueous Two‐Phase Systems (ATPS), simplifying the methodology and, potentially, reducing costs. This work aims to develop an economic contrast between ATPS and the traditional PEG precipitation method at different operation scales (10 to 1000 L bioreactor volume) to determine the applicability of the ATPS methodology at large scale. For this, the effect of ATPS volume ratio (V_R), sample loading, and materials discount over production cost were analyzed.

中文翻译：

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使用水性两相系统大规模生产M13噬菌体用于治疗应用的经济评估

噬菌体是生物纳米粒子，在基于生物技术的不同产品中有多种应用。其中，疫苗具有治疗抗生素耐药细菌和寄生虫感染的潜力。用于回收和纯化的传统方法依赖于用聚乙二醇（PEG）和NaCl沉淀。但是，由于大规模的技术限制，这种方法的适用性受到限制。最近，我们的研究小组使用两相水系统（ATPS）开发了噬菌体M13的回收和纯化策略，简化了方法，并有可能降低成本。这项工作的目的是在不同的操作规模（10至1000 L生物反应器体积）上建立ATPS与传统PEG沉淀方法之间的经济对比，从而确定ATPS方法在大规模应用中的适用性。为此，ATPS体积比的影响（V_R），样品装载量以及材料对生产成本的折扣进行了分析。