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Triangulation of methods using insect cell lines to investigate insecticidal mode-of-action.
Pest Management Science ( IF 4.1 ) Pub Date : 2020-08-19 , DOI: 10.1002/ps.6046
Michelle Mak 1 , Karren D Beattie 1 , Albert Basta 1 , David Randall 1 , Zhong-Hua Chen 1, 2 , Robert Spooner-Hart 2
Affiliation  

This study investigated three in vitro models to assist in elucidating possible mode‐of‐action, which could be adopted to evaluate insecticidal activity of complex, unknown, or multi‐constituent formulations. We used a combination of absorbance spectrometry, confocal scanning laser microscopy and microelectrode ion flux estimation (MIFE) to provide insight into potential target sites for insecticides. This study used two insect cell lines and evaluated three pyrethroid insecticides.

中文翻译:

使用昆虫细胞系研究杀虫作用模式的方法的三角剖分。

这项研究调查了三种体外模型,以帮助阐明可能的作用模式,这些模型可用于评估复杂,未知或多组分制剂的杀虫活性。我们将吸收光谱,共聚焦扫描激光显微镜和微电极离子通量估计(MIFE)结合使用,可深入了解杀虫剂的潜在目标部位。该研究使用了两种昆虫细胞系并评估了三种拟除虫菊酯类杀虫剂。
更新日期:2020-08-19
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