Abstract The evolution of next-generation neural interfaces toward high-density, high-fidelity, and long-term recordings coupled with the advent of complex fabrication techniques drives the development of 3D nanostructures to bridge the neuron-artificial device interface. This is largely due to several observations of high signal-to-noise ratios recorded from high-aspect-ratio 3D nanoelectrodes enabled by an inimitable mechanical coupling with the neural membrane and, in some cases, direct access to the neural intracellular space. This review details progress in developing 3D electrodes for electrogenic cell interfaces. First, the impact of nanotopography on the electrical and mechanical coupling of the cell and recording electrodes are discussed, and different conceptual approaches in understanding the biomechanics of cell/3D electrode coupling through experimental work and computational models are described. This work also highlights the latest advances in 3D nanostructures for recording the intracellular activity of cultured neurons and cardiomyocytes with a focus on emerging 3D structures and strategies aimed at improving efficiency and long-term integration with cultured electrogenic cells.

中文翻译：

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用于生电细胞胞内记录的三维纳米结构的进展

摘要 下一代神经接口向高密度、高保真度和长期记录的发展，加上复杂制造技术的出现，推动了 3D 纳米结构的发展，以桥接神经元 - 人工设备接口。这主要是由于对高纵横比 3D 纳米电极记录的高信噪比进行了多次观察，该电极通过与神经膜的独特机械耦合实现，在某些情况下，还可以直接进入神经细胞内空间。本综述详细介绍了开发用于生电细胞界面的 3D 电极的进展。首先，讨论了纳米形貌对细胞和记录电极的电气和机械耦合的影响，描述了通过实验工作和计算模型理解细胞/3D 电极耦合的生物力学的不同概念方法。这项工作还突出了 3D 纳米结构在记录培养神经元和心肌细胞的细胞内活动方面的最新进展，重点关注旨在提高效率和与培养的生电细胞长期整合的新兴 3D 结构和策略。