We introduce a new method for the analysis of enzyme-linked immunosorbent assay (ELISA) data. The new method can use data near the asymptotes and does not give undue weight to responses on the flatter parts of the dose-response curve. We apply it to simulated data and to two real-world assays and show it is more accurate and more precise than the traditional interpolation method. In particular, the new method works much better for very low-concentration samples for which the traditional method is often unable to give a result.

中文翻译：

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使用平行曲线分析评估ELISA结果。

我们介绍了一种用于分析酶联免疫吸附测定（ELISA）数据的新方法。该新方法可以使用渐近线附近的数据，并且不会在剂量反应曲线的较平坦部分上对反应给予过多的权重。我们将其应用于模拟数据和两个真实世界的分析，并显示它比传统的插值方法更准确，更精确。特别是，对于传统方法通常无法给出结果的非常低浓度的样品，新方法效果更好。