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Enhanced carotenoid production by Rhodopseudomonas palustris ATCC 17001 under low light conditions.
Journal of Biotechnology ( IF 4.1 ) Pub Date : 2020-08-20 , DOI: 10.1016/j.jbiotec.2020.08.007
J Lopez-Romero 1 , E Salgado-Manjarrez 2 , L Torres 1 , E I Garcia-Peña 1
Affiliation  

Carotenoids (CD) are biological pigments produced for commercial purposes. Therefore, it is necessary to study and determine the optimal conditions for increased CD production. There is no consensus in the literature about the conditions that increase CD production. Some authors stated that CD will be preferentially produced at low light intensities, at this adverse condition, microorganism will increase CD production as a survival response mechanism to get more energy. Other authors have mentioned that CD concentrations increase as the light intensity supplied increases, to avoid the overexposure of light and in turn photo-inhibition. Additionally, to increase the specific CD production is also necessary to increase the amount of biomass. In this work, the ammonium concentration (high (HAC) and low (LAC)) and the low light (LL) intensity effect on the CD production was evaluated. Data showed that a high CD-specific concentration of 8.8 mg/gcell was obtained by using R. palustris ATCC 17001 under HAC and LL intensity. CD production was similar at HAC and LAC, suggesting that the light intensity has a greater effect on the specific CD concentration than the nitrogen concentration. In general, the results showed a low biomass production compared to the literature, with high CD synthesis.



中文翻译:

沼泽红假单胞菌 ATCC 17001 在低光照条件下增强类胡萝卜素的产生。

类胡萝卜素 (CD) 是为商业目的生产的生物色素。因此,有必要研究和确定提高CD产量的最佳条件。关于增加 CD 产量的条件,文献中没有达成共识。有作者指出,在低光强下会优先产生CD,在这种不利条件下,微生物会增加CD产生作为生存反应机制以获得更多能量。其他作者提到 CD 浓度随着提供的光强度的增加而增加,以避免过度曝光,从而避免光抑制。此外,为了增加特定的 CD 产量,还需要增加生物量。在这项工作中,评估了铵浓度(高 (HAC) 和低 (LAC))和低光 (LL) 强度对 CD 产生的影响。数据显示,8.8 mg/g 的高 CD 特异性浓度在HAC和LL强度下使用R. palustris ATCC 17001获得细胞。HAC 和 LAC 的 CD 产量相似,表明光强度对特定 CD 浓度的影响大于氮浓度。总的来说,与文献相比,结果显示生物量产量低,CD 合成率高。

更新日期:2020-08-31
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