To clarify the physiological factors associated with the development of tomato leaf marginal necrosis associated with potassium deficiency, tomato leaf blades prior to development of the symptoms were collected, and profiles of water-soluble metabolites were analyzed using gas chromatography–mass spectrometry. Multivariate analysis was conducted to screen for a component that was related to potassium deficiency-induced leaf necrosis among the 60 metabolites detected in tomato leaves. Polyamines, sugars, and branched amino acids were ranked highly. Putrescine was most strongly and negatively correlated with potassium concentration and exhibited an exponential response, regardless of the site or plant body. In addition to putrescine, glucose, xylose and l-isoleucine are known to play important roles in stress response and ion balance control, and it is thought that changes in metabolite profiles are linked to tomato leaf marginal necrosis associated with a decrease in endogenous potassium concentration. These findings will be useful for cultivation management to mitigate this physiological disorder of tomato.

为了弄清楚与缺钾相关的番茄叶片边缘坏死的发展相关的生理因素，在症状出现之前收集番茄叶片，并使用气相色谱-质谱法分析水溶性代谢产物的概况。进行了多变量分析，以筛选与番茄叶片中检测到的60种代谢产物有关的钾缺乏引起的叶片坏死的相关成分。多胺，糖和支链氨基酸的排名很高。腐胺与钾浓度之间呈最强和负相关，并且无论其位点或植物体如何，均表现出指数响应。除腐胺，葡萄糖，木糖和升已知-异亮氨酸在应激反应和离子平衡控制中起重要作用，并且据认为代谢物谱的变化与番茄叶片边缘坏死有关，而后者与内源钾浓度的降低有关。这些发现将有助于减轻番茄的生理失调的栽培管理。