Cultivation of Agarophyton chilensis, common name “pelillo”, in Chile began 40 years ago. Technology adopted has been based on culture by vegetative propagation and culture from spores. Mass seaweed culture is associated with increased instances of pests and/or disease, as has been observed in the agricultural sector. The aim of this work is to study the pest alga Rhizoclonium sp, and its development under different controlled environmental conditions, given that this alga has affected “pelillo” production in southern Chile since 2015. Fresh thalli of the pest alga collected from culture centers located in Maullín river were described morphologically in order to confirm the identity of the species. Filaments were cultured under different temperature (10, 16 and 20 °C), photoperiod (8:16; 12:12 and 16:8 L:D), salinity (5; 10; 20; 27 and 30 psu) and nutrient (0.14 and 0.28 mmol/L nitrate) conditions to evaluate growth and reproduction. Nevertheless, given the taxonomic status situation of the polyphyletic genus Rhizoclonium, the pest is named Rhizoclonium-like. It formed lateral branches under culture conditions tested and showed a wide range of tolerance to temperature, salinity, photoperiod and nitrate concentration. Growth increases with temperature, reaching a daily growth rate of between 5.5 and 5.9%/day at 16 and 20 °C respectively. Rhizoclonium-like reproduction is described for the first time under culture conditions in Chile. Formation of reproductive structures occurs in the vegetative cells generating reproductive structures (zoosporangia) that mature under 16 °C, salinity >20 psu and nitrate concentration >0,14 mmol/L, observing release and germination of spores. After reproduction filaments do not decay and grow again.

智利的农杆菌（俗称“ pelillo”）栽培始于40年前。所采用的技术基于通过营养繁殖和孢子培养的培养。如在农业部门中观察到的，大规模海藻养殖与增加的病虫害和/或疾病有关。这项工作的目的是研究有害生物藻类Rhizoclonium sp，并且考虑到该藻类自2015年以来已影响智利南部“佩里洛”的生产，因此在不同的受控环境条件下进行了开发。对从位于毛利恩河养殖中心收集的有害藻类的新鲜藻类进行了形态学描述，以确认其身份。该物种。在不同温度（10、16和20°C），光周期（8:16; 12:12和16：8 L：D），盐度（5; 10; 20; 27和30 psu）和养分（ 0.14和0.28 mmol / L硝酸盐）条件以评估生长和繁殖。尽管如此，鉴于多系根瘤菌属的分类学状况，该害虫被称为根瘤菌-喜欢。它在测试的培养条件下形成侧枝，并表现出对温度，盐度，光周期和硝酸盐浓度的宽容度。生长随温度增加而增加，分别在16和20°C下达到每天5.5％至5.9％/天的日增长率。智利首次在培养条件下描述了根瘤菌样繁殖。生殖结构的形成发生在营养细胞中，该生殖细胞在16°C，盐度> 20 psu和硝酸盐浓度> 0.14 mmol / L的条件下成熟，观察到孢子的释放和萌发。繁殖后，细丝不会腐烂并再次生长。