A survey was conducted to determine the persistence of mycorrhization by Tuber melanosporum in truffle orchards established with European and American species of oak and common hazel trees in North Carolina. The trees had reportedly been inoculated and colonized by T. melanosporum prior to planting. Root samples were collected from 95 trees among seven orchards in 2015 and roots were analyzed by morphology and quantitative PCR. Samples that tested negative for T. melanosporum or where ectomycorrhizal morphology was not observed were analyzed by sequencing to identify the mycorrhizal fungal symbiont present. The presence of T. melanosporum was detected in all seven orchards. In six orchards, T. melanosporum was detected on all trees, but in only two of fifteen trees in one orchard. Other species of Tuber including T. brennemanii, T. canaliculatum, and T. lyonii, species of Scleroderma, and members of the Pezizales were also detected by sequence analysis. Sporocarps of T. aestivum and T. brumale were found in 2017 and 2018 in separate orchards in North Carolina after the survey was conducted. Overall, results indicate that T. melanosporum has persisted in truffle orchards sampled in North Carolina. Indigenous and contaminating fungal species, including Tuber species, were also detected and present a challenge to the truffle industry in North Carolina.

进行了一项调查，以确定在北美和北美洲的欧洲和美洲橡树和普通榛树种所建立的松露果园中，块茎黑色素菌对菌根的持久性。据报道，树木在播种前已被黑毛孢菌接种并定殖。2015年从7个果园的95棵树中收集了根样品，并通过形态学和定量PCR分析了根。通过测序分析检测出的T. melanosporum阴性或未观察到外生菌根形态的样品，以鉴定存在的菌根真菌共生菌。在所有七个果园中均检测到了黑色丝孢菌的存在。在六个果园中，黑毛锥虫在所有树木上均检测到，但在一个果园中只有15棵树木中有2棵被检测到。的其它物种的块茎包括T. brennemanii，T. canaliculatum，和T. lyonii，物种硬皮病也通过序列分析检测，并且盘菌目成员。孢子果普通小麦和T. brumale在2017年和2018年被发现在北卡罗来纳州的独立的果园进行调查以来之后。总体而言，结果表明，黑斑病菌在北卡罗来纳州采样的松露果园中持续存在。土著和污染性真菌物种，包括块茎 还发现了这些物种，这对北卡罗来纳州的松露业构成了挑战。