Apigenin is the bioactive component of parsley (Petroselinum crispum L.) with numerous therapeutic properties. In this study, microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE) methods were used for the isolation of apigenin from parsley leaves. The effect of several factors on the extraction yield of apigenin was investigated. In UAE method, under the best extraction condition (extraction solvent: ethanol 80%, time: 30 min, solid-to-solvent ratio: 1:25, particle size: 0.25 mm, ultrasonic power: 90%, frequency: 80 kHz and temperature: 40 °C) an extraction yield of 9.48 ± 0.11 mg apigenin/g parsley was achieved, while in MAE method, the best extraction condition (extraction solvent: ethanol 80%, time: 2 min, solid-to-solvent ratio: 1:20, particle size: 0.105 mm and microwave power: 180 W) resulted in a yield of 7.90 ± 0.14 mg apigenin/g parsley. Referring to Soxhlet extraction as a control (time: 6 h; temperature: 70 °C and solid-to-solvent ratio: 1:50), an apigenin extraction efficiency of 51.22 and 42.68% was obtained using UAE and MAE, respectively. Significantly lower extraction time, solvent consumption, and extraction temperature in UAE and MAE compared to those of Soxhlet method demonstrate the high efficiency of the modern extraction approaches. The UV–Vis and FTIR analyses confirmed that the structure of apigenin remained intact after extraction and purification. The purity of apigenin in the extracted sample from parsley leaves was 86.45% as determined by HPLC analysis.

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超声波和微波法从欧芹叶中提取芹菜素的比较研究

芹菜素是欧芹（Petroselinum crispum）的生物活性成分。L.）具有多种治疗特性。在这项研究中，微波辅助提取（MAE）和超声辅助提取（UAE）方法被用于从欧芹叶中分离芹菜素。研究了几种因素对芹菜素提取率的影响。在阿拉伯联合酋长国方法中，在最佳萃取条件下（萃取溶剂：乙醇80％，时间：30分钟，固溶剂比：1：25，粒径：0.25 mm，超声功率：90％，频率：80 kHz和温度：40°C）的提取产率为9.48±0.11 mg芹菜素/克欧芹，而在MAE方法中，最佳提取条件（提取溶剂：乙醇80％，时间：2分钟，固溶剂比： 1:20，粒径：0.105毫米，微波功率：180瓦），产量为7.90±0.14毫克芹菜素/克欧芹。以索氏提取为对照（时间：6 h；温度：70°C和固溶比：1:50），使用阿联酋和MAE获得的芹菜素提取效率分别为51.22和42.68％。与索氏萃取法相比，UAE和MAE中的萃取时间，溶剂消耗量和萃取温度显着降低，证明了现代萃取方法的高效率。UV-Vis和FTIR分析证实提取和纯化后芹菜素的结构保持完整。通过HPLC分析测定，芹菜叶提取的样品中芹菜素的纯度为86.45％。与索氏萃取法相比，阿联酋和阿拉伯联合酋长国的溶剂消耗量和萃取温度都证明了现代萃取方法的高效率。UV-Vis和FTIR分析证实提取和纯化后芹菜素的结构保持完整。通过HPLC分析测定，芹菜叶提取的样品中芹菜素的纯度为86.45％。与索氏萃取法相比，阿联酋和阿拉伯联合酋长国的溶剂消耗量和萃取温度都证明了现代萃取方法的高效率。UV-Vis和FTIR分析证实提取和纯化后芹菜素的结构保持完整。通过HPLC分析测定，芹菜叶提取的样品中芹菜素的纯度为86.45％。