Rhamnose is a naturally occurring deoxysugar present as a glycogenic component of plant and microbial natural products. A recombinant mutant Escherichia coli strain was developed by overexpressing genes involved in the TDP-L-rhamnose biosynthesis pathway of different bacterial strains and Saccharothrix espanaensis rhamnosyl transferase to conjugate intrinsic cytosolic TDP-L-rhamnose with anthraquinones supplemented exogenously. Among the five anthraquinones (alizarin, emodin, chrysazin, anthrarufin, and quinizarin) tested, quinizarin was biotransformed into a rhamoside derivative with the highest conversion ratio by whole cells of engineered E. coli. The quinizarin glycoside was identified by various chromatographic and spectroscopic analyses. The anti-proliferative property of the newly synthesized rhamnoside, quinizarin-4-O-α-L-rhamnoside, was assayed in various cancer cells.

中文翻译：

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大肠杆菌中鼠李糖基化蒽醌的生物合成。

鼠李糖是一种天然存在的脱氧糖，作为植物和微生物天然产物的糖原成分存在。通过过表达参与不同菌株的 TDP-L-鼠李糖生物合成途径的基因和Saccharothrix espanaensis鼠李糖基转移酶，将内源性胞质 TDP-L-鼠李糖与外源补充的蒽醌结合，开发了重组突变大肠杆菌菌株。在测试的五种蒽醌（茜素、大黄素、菊皂甙、蒽醌和醌茜）中，醌茜被工程化大肠杆菌全细胞生物转化为鼠李糖苷衍生物的转化率最高. 通过各种色谱和光谱分析鉴定了醌茜苷。在各种癌细胞中测定了新合成的鼠李糖苷 quinizarin-4-O-α-L-rhamnoside 的抗增殖特性。