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Water-Soluble Glycosylated Resveratrol for Measuring Residual Dipolar Coupling in NMR
Natural Product Communications ( IF 1.8 ) Pub Date : 2020-08-19 , DOI: 10.1177/1934578x20947232
Hiroki Hamada 1 , Masayoshi Yanagi 1 , Kei Shimoda 2 , Noriyuki Uchida 3
Affiliation  

Residual dipolar coupling (RDC) is a newly emerging technique for obtaining more precise conformational features of molecules in nuclear magnetic resonance (NMR), which can be observed when the molecules are oriented in NMR sample solutions. Unlike the case of solubilization of resveratrol (Res) in aqueous media by surfactants, multiglycosylated resveratrol (GlyRes) was dispersed in aqueous media forming nanoparticles with an extremely small size. In the presence of an NMR alignment medium of bacteriophage, GlyRes molecules were efficiently oriented in the strong magnetic field allowing for observations of RDCs in NMR. Structural refinement using the observed RDC values enabled differentiation of trans and cis forms of Res.



中文翻译:

水溶性糖基化白藜芦醇,用于在NMR中测量残留偶极偶合

残留偶极偶合(RDC)是一种用于在核磁共振(NMR)中获得分子更精确的构象特征的新兴技术,当分子在NMR样品溶液中取向时可以观察到。与表面活性剂溶解白藜芦醇(Res)在水性介质中的情况不同,多糖基化白藜芦醇(Gly Res)分散在水性介质中,形成尺寸非常小的纳米颗粒。在噬菌体的NMR定向介质存在下,Gly Res分子在强磁场中有效定向,从而可以在NMR中观察RDC。用观测RDC值结构细化启用分化反式RES的形式。

更新日期:2020-08-19
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